Table 2.

Effects of indole addition to conditioned medium from a tnaA mutant

StrainGrowth conditionaMean β-galactosidase activity ± SD (Miller units)b
MT113LB299 ± 6
CM (wild type)1,116 ± 57
CM (tnaAmutant)344 ± 17
CM (tnaA mutant) + 600 μM indole1,289 ± 39
CM (tnaA mutant) + 300 μM indole1,030 ± 79
CM (tnaA mutant) + 150 μM indole736 ± 11
MT114LB63 ± 2
CM (wild type)252 ± 8
CM (tnaAmutant)210 ± 10
CM (tnaA mutant) + 600 μM indole320 ± 12
CM (tnaA mutant) + 300 μM indole289 ± 16
CM (tnaA mutant) + 150 μM indole244 ± 12
  • a Conditioned medium (CM) was prepared at an A600 of = 1.4.

  • b Cells were harvested at anA600 of 0.35.