Table 3.

NADP and NAD concentrations and their redox state in cellular extractsa

StrainTreatmentNADPtotal (μmol of NADP/mg of Chlb)% NADP reducedNADtotal(μmol of NAD/mg of Chl)% NAD reduced
WTAir6.4 ± 0.1 (5.0 ± 0.3)75 ± 10 (70 ± 5)0.6 ± 0.2 (0.9 ± 0.1)65 ± 15 (55 ± 6)
SDH Defd Air2.5 ± 0.1 (2.1 ± 0.4)50 ± 15 (55 ± 6)0.1 ± 0.3 (0.4 ± 0.1)50 ± 21 (50 ± 5)
NDH-2 DefAir8.7 ± 0.1 (6.6 ± 0.1)35 ± 20 (25 ± 6)0.8 ± 0.2 (0.6 ± 0.1)100 ± 5 (100 ± 3)
WTc Air + 3% CO2 2.9 ± 0.1 (4.0 ± 0.4)50 ± 10 (50 ± 10)0.3 ± 0.4 (0.3 ± 0.1)70 ± 20 (45 ± 5)
NDH-1 DefAir + 3% CO2 0.5 ± 0.1 (0.8 ± 0.1)100 ± 4 (100 ± 3)0.02 ± 0.3 (0.1 ± 0.1)70 ± 20 (39 ± 9)
  • a The values reported are averages of three separate determinations using cells grown at 45 μmol of photons m−2 s−1. The standard deviations are indicated. Concentrations were determined by NAD and NADP cycling reactions and fluorescence-based HPLC (values in parentheses) as described in the text.

  • b Chl, chlorophyll.

  • c WT, wild type.

  • d Def, deficient.