Table 1.

Plasmids used in this studya

PlasmidDescriptionSource or reference
pBC SK(+)ColE1 ori; Cmr Stratagene
pHP45ΩkanPlasmid carrying Kmr cassette with transcriptional terminators at each end; Apr Kmr 14
pCR-Script Amp SK(+)ColE1 ori; Apr Stratagene
pMAL-c2 malEfusion protein expression vector; Apr New England Biolabs
pCP11 E. coli-F. johnsoniae shuttle plasmid; Apr (Emr)31
pCP23 E. coli-F. johnsoniae shuttle plasmid; Apr (Tcr)1
pLYL03 Bacteroides-Flavobacterium suicide vector used to make chromosomal insertions; Apr (Emr)29
pSA211.3-kb fragment containing gldA in pCP23; Apr (Tcr)1
pDH2331.9-kb fragment containing gldB in pCP23; Apr(Tcr)23
pMM1226-kb HindIII fragment of CJ282 DNA containing Tn4351 tetX gene and adjacent DNA in pBC SK(+); Cmr Tcr This study
pMM1294.6-kb SalI fragment containinggldD and adjacent DNA in pBC SK(+); Cmr This study
pMM1444.6-kb SalI fragment containinggldD in pCP23; Apr (Tcr)This study
pMM1663.6-kb SalI-Bst98I fragment containing fjo9, ssb, gldE, and gldD in pCP23; Apr (Tcr)This study
pMM1682.3-kbNsiI-Bst98I fragment containing gldEand gldD in pCP23; Apr (Tcr)This study
pMM1692.1-kb BglII-PstI fragment containing ssb and gldE in pCP23; Apr(Tcr)This study
pMM2091.8-kbClaI-Bst98I fragment containing gldDin pCP23; Apr (Tcr)This study
pMM210pMM209 with the Kmr cassette from pHP45Ωkan inserted upstream of gldD; Apr Kmr(Tcr)This study
pMM213Identical to pMM210 except that the Kmr cassette is inserted in the opposite orientation; Apr Kmr (Tcr)This study
pMM215607-bp ClaI-EcoRI fragment ofgldE in pLYL03; Apr (Emr)This study
pDH2470.9-kb fragment containing 3′ end ofgldE in SrfI site of pCR-Script SK(+); Apr This study
pDH249 gldE expression plasmid, 0.9-kb PstI-HindIII fragment of pDH247 in pMAL-c2; Apr This study
pDH2560.5-kb fragment containing 3′ end of gldD in SrfI site of pCR-Script SK(+); Apr This study
pDH263 gldD expression plasmid, 0.5-kbEcoRI-PstI fragment of pDH256 in pMAL-c2; Apr This study
  • a Antibiotic resistance phenotypes: ampicillin, Apr; chloramphenicol, Cmr; erythromycin, Emr; kanamycin, Kmr; tetracycline, Tcr. Unless indicated otherwise antibiotic resistance phenotypes are those expressed by E. coli. Antibiotic resistance phenotypes listed in parentheses are those expressed by F. johnsoniae but not by E. coli.