Table 1.

ORFs within the S. boydii aerobactin island

ORF, gene, or ISLocationLength (bp)Similar sequence (reference)% Nucleotide (protein) identity
int3 84–13451,261a P4 prophage integrase (37)(63)
shiB 1800–2249449 S. flexneri M90TshiB gene in SHI-2 (30)97 (94 over 143 of 153 aa)b
IS600 3198–44591,261 S. sonneiIS600 (10, 24)98
orf2 4815–5081266 S. flexneriSA100 orf24 in SHI-2 (43)100 (100)
rorf3c 5037–62291,192 S. flexneri SA100 rorf25 in SHI-2 (43)99 (89 for 297 of 397 aa)b,d
orf4 5877–6305428 S. flexneriSA100 orf27 in SHI-2 (43)99 (99)
iucA, -B, -C, -D 6308–121125,804 S. flexneri M90T aerobactin synthesis genes in SHI-2 (30)99 (99)
iutA 12115–143132,198 S. flexneriM90T iutA in SHI-2 (30)99 (99)
IS600 14542–158091,267 S. sonneiIS600 (10, 24)98 (93)
Partial IS285 15803–16159356 Y. pestisIS285(21)86 (79 for 88 of 270 aa)b
Partial IS200 16374–17106732 E. coli IS200 (4)95 (87)
ileX 17706–1777872 E. coli ileXtRNA gene (5)87
L0006 17928–18590662 E. coli LEE pathogenicity island prophage gene L0006 (37)93 (95)
L0005 18604–18786182 E. coli LEE pathogenicity island prophage gene L0005 (37)92 (78)
Partial L0004 18799–19047248 E. coli LEE pathogenicity island prophage gene L0004(37)90 (95 for 82 of 116 aa)b
IS600 19699–209611262 S. sonneiIS600 (10, 24)98 (93)
  • a Three ORFs corresponding to the integrase-like sequence were corrected for apparent frameshifts and are presented here as a single ORF.

  • b Amino acid identity for the partial ORF present in SHI-3.

  • c rorf3 is an ORF transcribed off the minus stand.

  • d Due to a premature stop codon,rorf3 is defective.