Table 1.

Bacteria and plasmids used

Strain or plasmidGenotype or descriptionaReference or sourceb
Strains
 AB259 supQ80λrelA1 spoT1 thi-1 CGSC
 SBG231AB259sjmA1; spontaneous MccJ25r mutantThis study
 SBG231recA SBG231 Δ(recA-srl)306 srl-301::Tn10-84 This study
 χ342 proC29 relA1 spoT1 metB1 bglF18::IS150 CGSC
 AB1133F thr-1 ara-14 leuB6 lacY1Δ(gpt-proA)62 supE44 galK2 λ rac hisG4 rfbD1 rpsL31 kdgK51 xyl-5 mtl-1 argE3 thi-1 CGSC
 SBG232SBG231 rpoB This study
 CSH126Δ(recA-srl)306 srl-301::Tn10-84 CSHL
Plasmids
 pDJJ11pBR322 carrying rplJL rpoB C. Gross
 pDJJ12pBR322 carrying rplJL rpoB rpoC htrC C. Gross
 pMADC2pDJJ12 carrying therpoC931 mutation (MccJ25r)This study
 pGC01pBR322 carrying fhuA J. Coulton
 pTUC347pUC18 carrying mcjABC; MccJ25+ImmJ25 30
 pJS200pACYC184 carryingmcjD; MccJ25 ImmJ25+ 29
 pGEM-4ZApr; cloning vectorPromega
  • a MccJ25r, resistant to microcin J25; MccJ25+, MccJ25 producer; ImmJ25+, immune to MccJ25.

  • b CGSC, E. coli Genetic Stock Center; CSHL, Cold Spring Harbor Laboratory.