Table 1.

Strains and plasmids used in this study

Strain or plasmidRelevant feature(s)Reference or source
Strains
M. xanthus
  DK1622Wild type19
  SW280 mrpB::Tn5-Ω280pilQ 15
  SW2800 mrpB::Tn5-Ω280This study
  SW2801 mrpB-lacZ201MrpB+ This study
  SW2802ΔmrpB This study
  SW2803 mrpC-lacZ202 mrpC + This study
  SW2804 mrpC-lacZ202 mrpC +ΔmrpB This study
  SW2806 mrpB-lacZ203 mrpB This study
  SW2807ΔmrpA This study
  SW2808ΔmrpC This study
  SW2811 mrpB-lac201 MrpB+ΔmrpC This study
  SW2841 mrpB D58E This study
  SW2844 mrpB D58A This study
 E. coli XL1-BlueHost for cloningStratagene
Plasmids
 pUC18Cloning vector, Ampr Gibco BRL
 pBlueScript KSCloning vector, Ampr Stratagene
 pBJ113In-frame deletion vector, Kanr Gals B. Julien and D. Kaiser
 pKY481 lacZ fusion vector, Kanr 7
 pSH112pUC18 carrying a 1.66-kb wild-type mrpB, template for site-directed mutagenesisThis study
 pSH113pBJ113 carrying anmrpB D58E mutationThis study
 pSH114pBJ113 carrying an mrpB D58A mutationThis study
 pSH201pKY481 carrying an mrpB-lac-201 translation fusionThis study
 pSH202pKY481 carrying anmrpC-lac-202 translation fusionThis study
 pSH203pKY481 carrying a mrpB-lac-203 translation fusionThis study
 pSH280pUC18 carrying a 26-kb DNA from SW2800This study
 pSH401pBJ113 carrying the deletedmrpB This study
 pSH402pBlueScript carrying wild-type mrpA This study
 pSH403pBJ113 carrying the deleted mrpA This study
 pSH404pUC18 carrying wild-type mrpC This study
 pSH405pBJ113 carrying the deleted mrpC This study