Table 2.

Synthetic oligonucleotides used in this study

Name Oligonucleotide sequencea
Oligonucleotides based on etrA
 E15′-CATAGCCCAGCGTCTTGTG-3′
 E25′-ACGATTATGCCAAATGACTTGAGT-3′
 E35′-ATTGCGATCGATGAACGTCTCGCCGCCTTTA-3′
 E45′-ATTGCGATCGATCCTTGTTCGGTGATGGTGTAACTT-3′
 E55′-CTTTGTATCCCGTTCACCCTCAAT-3′
 E65′-ATCCTAGCATTACCCGCCAAGA-3′
 E75′-GGCTGCCCTGTGGACTGGTA-3′
 E85′-CGAAGCGGTTAGCAAGGTTACTGA-3′
Oligonucleotide based on Kmrgene: K1  5′-ATTGCGATCGATTTATGCTTGTAAACC GTT-3′
Oligonucleotides based on cat gene
 C15′-CAGACGGCATGATGAACCTGAATC-3′
 C25′-CCACCGTTGATATATCCCAATGGC-3′
Oligonucleotides based on pCALc
 S15′-ATACGACTCACTATAGGGGAATTG-3′
 S25′-TGGCTGCTGAGACGGCTATGAAAT-3′
  • a Underlined regions indicate theClaI site engineered into oligonucleotides E3, E4, and K1.