Table 2.

β-Galactosidase activities conferred byARE1-lacZ and ARE2-lacZ fusions on wild-type strain BWG 1-7a

Plasmidb (promoter length, bp)β-Galactosidase activity in BWG1-7aa
ARE1-lacZARE2-lacZ
pIU1116 (1,000)87 + 3
pIU1141 (411)167 + 18
pIU1115 (1,000)1.2 + 0.2
pIU1160 (500)1.7 + 0.2
pIU1146 (220)1.7 + .3
  • a β-Galactosidase activity is expressed as nanomoles ofo-nitrophenyl-β-d-galactopyranoside hydrolyzed per minute per milligram of protein and is the average of two independent transformants assayed in duplicate over 3 days.

  • b The control plasmid lacking an AREpromoter insert typically gave a β-galactosidase activity of 0.5 U or less (21).