Table 3.

Quantification of sterols and sterol esters during overexpression of ARE1 in YEp3-16 (strain SCY059 [are1Δ are2Δ])

Compound(s)Free sterolsa (% of free compounds)Estersb (% of total esters)SE/FS ratioc
Lanosterol7.6 ± 14 (1.2)484 ± 159 (15.5)63
Zymosterol4.1 ± 8.6 (0.6)1,321 ± 284 (42.3)322
Fecosterol63 ± 9.2 (10.2)329 ± 70 (10.5)5
Episterol36 ± 9 (5.8)396 ± 120 (12.6)11
Ergosterol504 ± 125 (82.2)598 ± 199 (19.1)1
Total sterols614 ± 138 (100)3,128 ± 798 (100)5
  • a Sterols (micrograms per gram [wet weight] of cells ± the standard deviation) from three separate cultures were extracted, and the average value is reported.

  • b Mass of sterols (micrograms per gram [wet weight] of cells ± the standard deviation) in the esterified form after saponification (three separate cultures were extracted, and the average value is reported).

  • c The SE/FS ratio represents the proportion of steryl ester (SE) relative to that remaining unesterfied (FS).