Table 4.

Quantification of sterols and steryl esters during overexpression of ARE2 in Yep352Are2 (strain SCY059 [are1Δ are2Δ])

Compound(s)Free sterolsa (% of free compounds)Estersb (% of total esters)SE/FS ratioc
Lanosterol1.8 ± 4.9 (0.3)22.9 ± 8.6 (0.8)13
Zymosterol24 ± 27 (4.7)1,029 ± 249 (34.9)43
Fecosterol56.4 ± 14 (11.1)467 ± 143 (15.8)8
Episterol47 ± 24 (9.2)289 ± 96 (9.8)6
Ergosterol380 ± 198 (74.7)1,140 ± 302 (38.7)3
Total sterols509 ± 251 (100)2,948 ± 714 (100)6
  • a Sterols (micrograms per gram [wet weight] of cells ± the standard deviation) from three separate cultures were extracted, and the average value is reported.

  • b Mass of sterols (micrograms per gram [wet weight] of cells ± the standard deviation) in the esterified form after saponification (three separate cultures were extracted, and the average value is reported).

  • c The SE/FS ratio represents the proportion of steryl ester (SE) relative to that remaining unesterfied (FS).