Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotype and/or descriptionSource
Strains
E. coli
  DH5αF′F′endA1 hsdR17 supE44 thi-1 recA1 gyrA relA1 φ80d lacZΔM15 (lacZYA-argF)U169 Life Technologies
B. suis
  1330Biotype 1; ATCC 23444ATCC
  1330 ΔchvEbs B. suis 1330 ΔchvEbs::kan(chvEbs null mutant)This work
  1330 ΔgguAbs B. suis 1330 ΔgguAbs::kan (gguAbs null mutant)This work
  1330 ΔnikA B. suis 1330 Δnika::kan 23
B. melitensis 16 MBiotype 1; ATCC 23456ATCC
B. abortus
  A1Biotype 1; ATCC 23448ATCC
  A3Biotype 3; ATCC 23450ATCC
B. canis ATCC 23365ATCC
B. ovis ATCC 25840ATCC
A. tumefaciens
  C58Wild-type, pTiC58 (nopaline)22
  A348Chromosomal C58, pTiA6 NC (octopine)41
  MX1C58;chvE::Tn5 mutant with pSM243cd22
Plasmids
 pUC18ColE1 replicon,lacZ (Ampr)AP Biotech
 pUC4KContains a Kanr cassette flanked by polylinkerAP Biotech
 pCVD442Vector with sacBencoding sucrose sensitivity (Ampr)14
 pBBRIMCSBroad-host-range cloning vector (Cmr)27
 pUC18::chvEbs pUC18 containing a 2.7-kbHindIII fragment of B. suis encoding the completechvEbs gene and part of gguAbs(Ampr)This work
 pUC18::gguAbs pUC18 containing a 4.5-kbHincII fragment of B. suis encoding part ofchvEbs and the complete gguAbs, gguB, and orf4 genes (Ampr)This work
 pUC18 ΔchvEbs::kan pUC18 derivative withchvEbs null allele in NruI siteThis work
 pUC18 ΔgguAbs::kan pUC18 derivative with gguAbs null allele in EcoRV siteThis work
 pCVD ΔchvEbs::kan pCVD442 withchvEbs null allele inNdeI-EcoRVThis work
 pCVD ΔgguAbs::kan pCVD442 withgguAbs null allele inNcoI-HindIIIThis work
 pBBR1::chvEbs pBBR1MCS derivative with the chvEbs gene inHindIII-NcoIThis work
 pBBR1::chvEbs2 pBBR1MCS derivative with the chvEbs gene inHindIII-EcoRVThis work
 pBBR1::gguAbs pBBR1MCS derivative with the gguAbs, gguB, and orf4 genes inPstI-SacIThis work
 pBBR1::chvEbsoperon pBBR1MCS derivative with the chvEbsoperon and orf4 genes inHindIII-SacIThis work
 pBBR1::gbpR-chvE pBBR1MCS derivative with the gbpR-chvE gene from A. tumefaciens inEcoRIThis work
 pSM243cd virB::Tn3-HoHo1 (virB::lacZ fusion, Cbr)44
 pTC110IncW, derivative of pUCD2 (Kanr; Gmr)9
 pTC110::chvEbs pTC110 derivative with thechvEbs gene in HindIIIThis work
 pTC116pTC110 derivative with the gbpR-chvE gene inEcoRI9