Table 1.

Bacterial strains, plasmids, and oligonucleotides

Strain, plasmid, or oligonucleotideDescriptionReference
Strains
P. putida
  PaW85Tn4652 3
  PKS54Tn4652 rpoS::Km20
Plasmids
 pAYC32Broad-host-range vector (Apr)6
 pEST1332Plasmid pAYC32 carrying promoterless pheBA operon15
 pKT240Cloning vector (Apr Kmr)2
 pKTtnpA(D/H)Tn4652 tnpA gene cloned into pKT24012
 pKTlacZS/C122-bp Tn4652 tnpA promoter region with IHF binding site cloned into pKTlacZ11
 pKTlacZD/C65-bp Tn4652 tnpA promoter region lacking IHF binding site cloned into pKTlacZ11
Oligonucleotides
 pheA5′-TGCTCAAGATTATCATTACGCT-3′ (positions 11–32 in the pheA coding region)
 TnR5′-ATCAGCATAGACGGCTAGCCAG-3′ (positions 101–122 from Tn4652 right end)
 TnL5′-CTTCCTCAATGGATGGCTGAAG-3′ (positions 111–132 from Tn4652 left end)