Table 3.

Complementation of therhlAB::lacZ fusion with C4-HSL and PQS

Signal addedβ-Gal activitiesa (Miller units)
PT5 (pECP60)PT149 (pECP60)PT464 (pECP60)
None23,298 ± 4,318268 ± 10168 ± 45
C4-HSL (10 μM)21,512 ± 2741,716 ± 484133 ± 24
PQS (50 μM)16,258 ± 3,453249 ± 4103 ± 11
C4-HSL (10 μM) + PQS (50 μM)21,058 ± 4,6744,073 ± 146129 ± 24
  • a Cultures were grown for 3 h in the absence of signal; after signal was added, cultures were incubated for 18 h and β-galactosidase (β-Gal) activity was determined. Values are means from two independent experiments done in triplicate.