Table 2.

Exoproduct assays and resistance profiles in P. aeruginosa multidrug efflux and quorum-sensing mutants

StrainGenotypeExoproduct activityaMIC of:
PyocyaninElastaseCiprofloxacinChloramphenicolImipenem
PT5wtb1001000.125321
PT149nfxC54211,0244
PT454rhlI3ND0.125321
PT462rhlR<140.125321
PT466lasI80ND0.125641
PT498lasR4560.125321
PT531rhlR lasR<140.125321
PT637nfxC mexEND880.125324
PAO-BInfxCND4511,0244
PAO-RInfxC lasRND<111,0244
  • a Expressed as a percentage of the activity in the wild-type strain PT5. Pyocyanin activities were determined in supernatants of cultures grown for 18 h in PB medium. ECR assays were performed on supernatants of cultures grown for 10 h in PB medium. At this point the cultures were in stationary phase, and the OD600s of the cultures were 5 ± 0.5. ND, not determined.

  • b wt, wild type.