Table 7.

Induction of the ςW regulon in B. subtilis by salt or ethanol stressa

GeneFunction or nearest homolog (E value)Regulatory region or potential promoter sequencesInduction ratio
wtsigB
EtOHSaltEtOHSalt
xpaC5-Bromo-4-chloro-indolyl phosphate hydrolysis proteinAAGATGAAACTTGTTTAAGGATTGAACGTAGTAG-N41-ATG1.42.31.62.3
yaaNTellurite resistance protein TelA, R. sphaeroides (2e-50)xpaC yaaNoperon1.44.72.25.7
sigWRNA polymerase ECF type sigma factorAAATTGAAACCTTTTGAAACGAAGCTCGTATACATACA(GA)C1.33.31.43.7
ybbMAnti-ς factorsigW ybbMoperon1.24.22.78.6
yceCResistance protein CdrC, C. acetobutylicum(2e-43)TTTACGAAACTTTGATATAATAACAAACGTATATA-N83-GTG2.99.66.11.2
yceDResistance protein CdrC, C. acetobutylicum (7e-63)Potential yceCDEFGHoperon3.26.85.78.8
yceEResistance protein CdrC, C. acetobutylicum (1e-74)PotentialyceCDEFGH operon2.76.28.31.2
yceFToxic anion resistance protein YkoY, B. subtilis (4e-28)Potential yceCDEFGHoperon2.15.24.07.3
yceGNo similarityPotential yceCDEFGHoperon2.76.84.17.9
yceHTellurite resistance protein TelA, R. sphaeroides (1e-30)PotentialyceCDEFGH operon1.74.93.69.6
ydbSConserved protein, B. halodurans(3e-28)AGAATGAAACCTTTCTGTAAAAGAGACGTATAAATAA(CG)A2.16.72.85.3
ydbTConserved protein BH1721, B. halodurans (9e-68)ydbSToperon1.47.12.24.9
ydjFPhage shock protein A DR1473, D. radiodurans(4e-20)AAAGTGAAACTTTTAACGATAATAAATAGTATATG-N40-ATG2.39.13.914
ydjGUnknown protein BH1806, B. halodurans(6e-74)ydjFGHI operon1.25.52.49.6
ydjHUnknown protein BH1807, B. halodurans(2e-11)ydjFGHI operon1.53.01.84.5
ydjIUnknown protein BH1805, B. halodurans(4e-5)ydjFGHI operon0.94.11.77.8
ydjONo similarityyeaA ydjPOoperon1.04.21.05.3
ydjPBromide peroxidase, S. aureofaciens (1e-18)yeaA ydjPOoperon1.85.91.45.9
yeaANo similarityTTTATGAAACCTTTGGCCCTATTTATCGTATTACGT(A)AAA1.44.81.24.8
yfhLHypothetical protein YvaZ, B. subtilis(5e-05)TGCATGAAACATTTCTTCTTTCTGCACGTAACAATGA(GA)A5.33.91.62.0
yfhMEpoxide hydrolase-related protein, D. radiodurans (9e-61)yfhLMoperon9.87.53.84.1
yjoBFtsH,H. pylori(2e-20)GGGATGAAACAAAATGCTATGTCAATCGTATATATAAC(G)T3.6152.213
yknWUnknown conserved protein, B. halodurans(4e-20)AACATGAAACTTTTTGATATCCTTCCCGTACTATTTGT(T)A1.07.52.411
yknXbABC-transporter-like protein TptB, S. cristatus (5e-23)yknWXYZoperon0.40.70.90.6
yknYPutative ABC transporter YvrO, B. subtilis (3e-75)yknWXYZoperon1.23.43.25.5
yknZABC transporter–ATP-binding protein NMB0549, Neisseria meningitidis (6e-52)yknWXYZoperon1.22.82.33.8
yndNGlutathione transferase FosB (EC 2.5.1.18 ), S. epidermidis(8e-48)TGTATGAAACTTTCTTATGAAAAAAGTCGTATATG-N32-GTG1.34.52.26.4
yoaFNo similarityATAATGAAACCCGGAGTATGCCAAGCCCGTATAAC-N28-ATG0.33.20.76.4
yobJNo similarityTATATGAAACCTTTTTTATTTTAGCCCGTATTAAAAGT(A)A1.25.23.910
yozOHypothetical protein YjqA, B. subtilis(7e-10)ATATTGAAACTTTTTTCTCTATATGTGCGTATTAC-N171-TTG0.66.10.66.4
yqfBNo similarityyqeZ yqfABoperon1.23.91.66.2
yqfAORF1,B. megaterium (1e-151)yqeZ yqfABoperon1.7242.822
yqeZConserved protein BH1356, B. halodurans(e-103)AAAATGAAACCTTTGATACATTTGTTACGTATGAA-N42-TTG1.3171.817
yteIProteinase IV, A. aeolicus(1e-37)GAAGTGAAACATTTTTCATATTGAATCGTATAATGAG(AG)A2.43.65.34.7
ythQABC transporter (permease) EcsB, B. halodurans (6e-05)ythPQoperon1.53.42.34.7
ythPABC transporter–ATP-binding protein EcsA, B. subtilis(2e-45)TTAAAGAAACTTTTTTTATTCTATTTCGTAGTAA-N20-TTG1.32.51.32.6
yuaIProbable acetyltransferase, D. radiodurans (1e-15)yuaFGIoperon2.5347.062
yuaGEpidermal surface antigen, B. halodurans (4e-78)yuaFGIoperon3.6577.268
yuaFNo similarityATTTTGAAACTTTTCCCGAGGTGTCTCGTATAAATGGT(A)A1.7142.720
yvlDUnknown conserved protein, B. halodurans (4e-23)yvlABCDoperon1.56.02.79.3
yvlCConserved protein BH3592, B. halodurans (3e-11)yvlABCDoperon0.92.31.22.9
yvlBUnknown conserved protein, B. halodurans(4e-63)yvlABCD operon2.77.23.411
yvlANo similarityAATTTGAAACCTGAAGAGATTTTAAACGTATAAATAA(GT)A2.06.13.19.2
ywrENo similarityTTTATGAAACGTTTTTCCTTTTTCTTCGTATAAAGGTA(GA)0.72.40.73.5
  • a See Table 1, footnotes a through e, and Table 2, footnote b, for explanations of data presentation.

  • b Operon internal genes that do not meet our stringent expression criteria are included here if the flanking genes display ςB-dependent expression or if the operon structure has been proven by Northern blot analysis.