Table 1.

Strains used in this study and some associated characteristics

Strain no.SerovaraResistance profilebIsolation siteSGI1 junction PCR resultscXbaI fragments(s) hybridizing withd:Reference
LeftRightqac/sulIp1–9
96-5227DT104ACSSuTCanada++11.7, 4.39, 424
BN3791DT104ACSSuTFrance++11.7, 4.39, 4This work
1641SA96DT104ACSSuTBelgium++11.7, 4.39, 4This work
1276SA96DT104ACSSuTBelgium++11.7, 4.39, 4This work
1390SA96DT104ACSSuTBelgium++11.7, 4.39, 4This work
2019SA96DT104ACSSuTBelgium++11.7, 4.39, 4This work
S/952569DT104ACSSuTScotland++NDe NDe This work
S/960081DT104ASSuTScotland++99, 4This work
S/960725DT104ASuScotland++4.39, 4This work
S/954435DT104SSuScotland++79, 4This work
S/921495DT104SensitiveScotlandNoneNoneThis work
424SA93DT120ACSSuTBelgium++11.7, 4.39, 47
1439SA96DT120ACSSuTBelgium++11.7, 4.39, 47
959SA97AgonaACSSuTBelgium++11.7, 4.39, 47
251SA97AgonaACSSuTBelgium++11.7, 8.49, 47
1169SA97AgonaACSSuTBelgium++11.7, 8.49, 4This work
1146SA97AgonaACSSuTBelgium++11.7, 8.49, 4This work
0059SA98AgonaSensitiveBelgiumNoneNoneThis work
  • a S. enterica serovars Typhimurium DT104, Typhimurium DT120, and Agona.

  • b A, ampicillin; C, chloramphenicol; S, spectinomycin and streptomycin; Su, sulfonamides; T, tetracycline.

  • c +, product obtained; −, no product obtained. Left, U7-L12 and LJ-R1 primers; right, 104-RJ and C9-L2 or 104-RJ and 104-D primers.

  • d The qac/sulI probe was an amplicon generated with QS-1 and QS-2 primers; p1–9 is a 2-kb EcoRI fragment (see Fig. 1) cloned in pBluescript II.

  • e ND, not done.