Table 1.

Strains and plasmids used

Strain or plasmidGenotypeSource or reference
Strains
 14028Wild-typeSalmonella serovar TyphimuriumAmerican Type Culture Collection
 BA61214028sdiA1::mTn3 (Ampr)1
 UT481E. coli K-12 Δlac53
 WX2UT481 sdiA::kan53
 BA110114028 srg-5::MudJ1
 BA110214028 srgB2::MudJ1
 BA110314028 srgA1::MudJ1
 BA110414028 rck2::MudJ1
 BA110514028 rck3::MudJ1
 BA110714028 srgB1::MudJ1
 BA110914028 srgC1::MudJ1
 BA111014028 srg-6::MudJ1
 BA111114028 rck1::MudJ1
 BA111214028 srg-7::MudJ1
 BA130314028 srgA1::MudJsdiA::mTn31
Plasmids
 pWSK29pSC101 cloning vector (Ampr)52
 pWSK129pSC101 cloning vector (Kanr)52
 pBA306pWSK29 Salmonellaserovar Typhimurium sdiA+(Ampr)1
 pBM1pWSK129 Salmonellaserovar Typhimurium sdiA+(Kanr)This study
 pCX39Mini-F′ carryingftsQAZ promoter 2 (sdiA sensitive) fusion togalK′-lacZYA; Ampr53
 pJVR2sdiA under control of araBADpromoter; pACYC origin; Cmr1
 pBA321sdiA under control of araBADpromoter; ColE1 origin; KanrThis study
 pSB401luxR+luxI::luxCDABE; pACYC origin; Tetr57
 pBA401pSB401ΔEcoRI carrying ORF7-srgA intergenic regionThis study
 pBA403pSB401ΔEcoRI carrying ORF6-pefIintergenic region, pefI, and ORF7This study
 pBA405pSB401ΔEcoRI carrying ORF6-pefIintergenic region and pefIThis study
 pBA428pSB401ΔEcoRI carrying ORF6-pefIintergenic regionThis study