Table 1.

Bacterial strains and plasmids used

Strain or plasmidRelevant characteristicsReference or source
Strains
S. flexneri
  YSH6000Wild-typeS. flexneri 2a Japanese isolate; SmrApr CmrTcr 46
  YSH6000TMRDE deletant of YSH6000; Sms Aps CmsTcs 38
  S2430MRDE deletant of YSH6000; Sms Aps Cms Tcs 39
  SBA1304Wild-type S. dysenteriae 3 Japanese isolateThis study
  SBA1363Spontaneous SRL PAI deletant of YSH6000; Sms Aps CmsTcs This study
  SBA1365Spontaneous MRDE deletant of YSH6000; Sms Aps CmsTcs This study
  SBA1366Spontaneous SRL deletant of YSH6000; Sms Aps CmsTcs This study
  SBA1367Spontaneous SRL PAI deletant of YSH6000; Sms Aps CmsTcs This study
  SBA1368Spontaneous SRL deletant of YSH6000; Sms Aps CmsTcs This study
  SBA1369Spontaneous MRDE deletant of YSH6000; Sms Aps CmsTcs This study
  AL11YSH6000 insertionalint mutant; Smr Apr CmrTcr Knr This study
  AL108YSH6000 harboring pBRTprΔ; Smr AprCmr Tcr Tpr This study
  AL109AL11 harboring pBRTprΔ; SmrApr Cmr Tcr KnrTpr This study
  AL110AL11 harboring pAL66; Smr Apr Cmr TcrKnr Tpr This study
E. coli
  DH5αFφ80dlacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdR17 Deor thi-1 sunE441 gyrA96 relA1 Bethesda Research Laboratories
  S17-1(λpir)RP4-2(Tcs::Mu)(Kns::Tn7)recA λpir Smr Tpr 36
  JM109(λpir) recA1 supE44 endA1 hsdR17 gyrA96 relA1 thi Δ(lac-proAB) λpir 36
Plasmids
 pWSK29pSC101-based low-copy-number vector; ΔlacZ Apr 47
 pWSK129pSC101-based low-copy-number vector; ΔlacZ Knr 47
 pBRTprΔ4.8-kb pBR322-based cloning vector; Tpr Tcr C. Sasakawa
 pJP5603R6K-based suicide vector; Knr 40
 pSBA348pWSK29 harboring 23-kb BamHI fragment containing solA-mdoA region of YSH6000K. Rajakumar
 pSBA509pWSK129 harboring part of SRL PAI and flanking regionsS. N. Luck
 pSBA533pWSK129 harboring 14-kbEcoRI subclone from pSBA509S. N. Luck
 pSBA574pWSK29 harboring SBA1366 SRL deletion region; Apr This study
 pSBA575pWSK29 harboring SBA1368 SRL deletion region; Apr This study
 pAL11pJP5603 harboring 666-bp internal SRL PAI integrase gene fragmentThis study
 pAL64pWSK29 harboring 1.3-kb PCR fragment containing int This study
 pAL66pBRTprΔ harboring 1.3-kb intfragmentThis study