Table 1.

B. subtilis strains used in this study.

StrainGenotypeaSource
168 trpC2 BGSCb
BG4233ΔmtrB argC4 15
CB312 his met C. Stewartc
CB313 sup-3 C. Stewart
PLBS127 rpoB18 amyE::[trpp(−412 to +203)trpE′-′lacZ Cmr]11
PLBS129 rpoB18 amyE::[trpp(−412 to +203) SDm o d trpE′-′lacZ Cmr]11
PLBS138 his met amyE::[trpp(−412 to +203)trpED′-′lacZ (trpE stop) Cmr]This study
PLBS139 sup-3 amyE::[trpp(−412 to +203)trpED′-′lacZ (trpE stop) Cmr]This study
PLBS142 his met amyE::[trpp(−412 to +203)trpED′-′lacZ Cmr]This study
PLBS143 sup-3 amyE::[trpp(−412 to +203) trpED′-′lacZ Cmr]This study
PLBS144 his met amyE::[trpp(−412 to +203) SDm o d trpED′-′lacZ (trpE stop) Cmr]This study
PLBS145 sup-3 amyE::[trpp(−412 to +203) SDm o d trpED′-′lacZ (trpE stop) Cmr]This study
PLBS146 his met amyE::[trpp(−412 to +203) SDm o d trpED′-′lacZ Cmr]This study
PLBS147 sup-3 amyE::[trpp(−412 to +203) SDm o d trpED′-′lacZCmr]This study
PLBS176 trpC2 amyE::[trpp(−412 to +203)trpE′-′lacZ Cmr]This study
PLBS201 trpC2 amyE::[trpp(−412 to +203) SDm o d trpE′-′lacZCmr]This study
PLBS283ΔmtrB argC4 his met amyE::[trpp(−412 to +203)trpED′-′lacZ (trpE stop) Cmr]This study
PLBS284ΔmtrB argC4 sup-3 amyE::[trpp(−412 to +203)trpED′-′lacZ (trpE stop) Cmr]This study
PLBS285ΔmtrB argC4 his met amyE::[trpp(−412 to +203)trpED′-′lacZ Cmr]This study
PLBS286ΔmtrB argC4 sup-3 amyE::[trpp(−412 to +203)trpED′-′lacZ Cmr]This study
PLBS289 rpoB18 rho::neo amyE::[trpp(−412 to +203)trpE′-′lacZ Cmr]This study
PLBS291 rho::neo his met amyE::[trpp(−412 to +203)trpED′-′lacZ (trpE stop) Cmr]This study
PLBS292 rho::neo sup-3 amyE::[trpp(−412 to +203)trpED′-′lacZ (trpE stop) Cmr]This study
PLBS293 rho::neo his met amyE::[trpp(−412 to +203)trpED′-′lacZ Cmr]This study
PLBS294 rho::neo sup-3 amyE::[trpp(−412 to +203)trpED′-′lacZ Cmr]This study
PLBS321 rho::neo his met This study
PLBS322 rho::neo trpC2 This study
W168 rpoB18 BGSC
  • a trpp denotes thetrp promoter; a prime indicates truncation of the gene; −412 to +203 is the DNA fragment containing trpp and neighboring regions that was incorporated, relative to the transcription start site; (trpE stop) indicates that the strain contains an engineered UAA stop codon in the trpEcoding sequence. trpED′-′lacZ contains an in-frame deletion of trpE as well as a translational fusion betweentrpD and lacZ. sup-3 encodes a UAA tRNA suppressor.

  • b BGSC, Bacillus Genetic Stock Center, Ohio State University, Columbus, Ohio.

  • c These strains were obtained from Charles R. Stewart, Department of Biochemistry and Cell Biology, Rice University, Houston, Tex.