Table 5.

Effect of Rho on trpE and trpDtranscript levels

StrainRelevant genotypeTryptophan (μM)mRNA level (%)a
trpEtrpD
168 trpC2 2051 ± 653 ± 6
168 trpC2 2005.0 ± 0.34.9 ± 0.4
PLBS322 rho::neo trpC2 20100100
PLBS322 rho::neo trpC2 20043 ± 237 ± 7
CB312WT014 ± 119 ± 5
CB312WT2005.0 ± 1.03.4 ± 0.6
PLBS321 rho::neo 0100100
PLBS321 rho::neo 20048 ± 144 ± 4
  • a The highest level of bothtrpE and trpD mRNAs was present in rhomutant strains grown under tryptophan-limiting conditions and was set at 100%. The values are averages of two independent experiments ± standard deviation.