Table 2.

Growth and sporulation of class A PBP mutant strainsa

StrainPBP phenotypeDoubling timeb (min)Cell countsc (CFU/ml)
ViableHeat-resistant sporesChloroform-resistant spores
PS832Wild type202 × 109 2 × 109 2 × 109
PS1869PBP2c 201 × 109 2 × 109 2 × 109
DPVB45PBP2d 204 × 109 2 × 109 2 × 109
DPVB42PBP4 203 × 109 2 × 109 1 × 109
PS2062PBP1 252 × 109 3 × 109 2 × 109
DPVB57PBP4PBP2d 201 × 109 7 × 108 9 × 108
DPVB56PBP2cPBP2d 202 × 108 4 × 104 2 × 104
DPVB46PBP2cPBP4 202 × 109 4 × 108 7 × 108
DPVB61PBP1PBP2d 262 × 109 2 × 109 2 × 109
PS2251PBP1PBP2c 267 × 108 1 × 109 7 × 108
DPVB68PBP1PBP4 315 × 108 3 × 107 4 × 107
DPVB49PBP2c PBP4PBP2d 218 × 108 7 × 103 3 × 102
DPVB63PBP1 PBP2cPBP2d 284 × 108 8 × 103 5 × 103
DPVB69PBP1 PBP2cPBP4 284 × 108 9 × 106 5 × 107
DPVB62PBP1 PBP4PBP2d 314 × 108 2 × 107 1 × 107
  • a Doubling times and cell counts are averages from at least three separate experiments.

  • b Growth was in liquid 2 × SG medium at 37°C.

  • c Cell counts determined 24 h after initiation of sporulation.