Table 1.

B. subtilis strains used

StrainGenotypeaTransformationSource or reference
Donor(s)Recipient
IA626 hisA82::Tn917 Bacillus Genetic Stock Center
AD51 gerE-lacZ 11
AD52 cotD-lacZ 58
AD799 sspB-lacZ 26
DPVB29Double ΔpbpD::CmpDPC271PS832This work
DPVB30ΔpbpD (Spo)This work
DPVB40ΔpbpD hisA82::Tn917(Spo+)1A626DPVB30This work
DPVB42ΔpbpD(Spo+)DPVB30DPVB40This work
DPVB45ΔpbpG::KnpDPV35PS832This work
DPVB46ΔpbpDΔpbpF::Ermr PS1869DPVB42This work
DPVB49ΔpbpDΔpbpG::KnΔpbpF::Ermr DPVB45DPVB46This work
DPVB56ΔpbpG::KnΔpbpF::Ermr DPVB45PS1869This work
DPVB57ΔpbpDΔpbpG::KnDPVB45DPVB42This work
DPVB61ΔponA::Sp ΔpbpG::KnPS2062DPVB45This work
DPVB62ΔponA::Sp ΔpbpDΔpbpG::KnPS2062DPVB57This work
DPVB63ΔponA::Sp ΔpbpG::Kn ΔpbpF::Ermr PS2062DPVB56This work
DPVB68ΔponA::Sp ΔpbpD PS2062DPVB42This work
DPVB69ΔponA::Sp ΔpbpDΔpbpF::Ermr PS2062DPVB46This work
DPVB84 sspB-lacZ AD799PS832This work
DPVB85 sspB-lacZΔpbpF::Ermr AD799PS1869This work
DPVB86 sspB-lacZΔpbpG::KnAD799DPVB45This work
DPVB89 sspB-lacZ ΔpbpG::Kn ΔpbpF::Ermr DPVB84DPVB56This work
DPVB90 gerE-lacZ AD51PS832This work
DPVB91 gerE-lacZΔpbpF::Ermr AD51PS1869This work
DPVB92 gerE-lacZΔpbpG::KnAD51DPVB45This work
DPVB93 gerE-lacZ ΔpbpG::Kn ΔpbpF::Ermr AD51DPVB56This work
DPVB94 cotD-lacZ AD52PS832This work
DPVB95 cotD-lacZΔpbpF::Ermr AD52PS1869This work
DPVB96 cotD-lacZΔpbpG::KnAD52DPVB45This work
DPVB97 cotD-lacZ ΔpbpG::Kn ΔpbpF::Ermr AD52DPVB56This work
DPVB141ΔpbpG::KncwlD::CmPS2307DPVB45This work
DPVB142ΔpbpF::Ermr cwlD::CmPS2307PS1869This work
DPVB143ΔpbpG::Kn ΔpbpF::ErmcwlD::CmPS2307DPVB56This work
DPVB157ΔpbpF::ErmsleB::SpcwlJ::Tetr FB111, FB112PS1869This work
DPVB158ΔpbpG::KnsleB::SpcwlJ::Tetr FB111, FB112DPVB45This work
DPVB159ΔpbpG::Kn ΔpbpF::Ermr sleB::SpcwlJ::Tetr FB111, FB112DPVB56This work
FB111 cwlJ::Tetr 33
FB112 sleB::Sp33
FB113 cwlJ::Tetr sleB::Sp33
PS832Prototrophic revertant of strain 168Laboratory stock
PS1869ΔpbpF::Ermr pDPC89PS83242
PS2062ΔponA::SppDPC197PS83241
PS2251ΔponA::Sp ΔpbpF::Ermr PS2062PS186944
PS2307 cwlD::CmADD1PS83245
  • a Abbreviations: Ermrresistance to erythromycin and lincomycin; Sp, resistance to spectinomycin; Cm, resistance to chloramphenicol; Kn, resistance to kanamycin; Tetr, resistance to tetracycline.