Table 1.

New mrcA (PBP 1a) mutantsa

StrainPBPs DeletedParentStrainPBPs DeletedParentStrainPBPs DeletedParent
CS229-1K1a 7CS9-19CS456-1K1a 4 5 7CS315-1CS541-1K1a 4 7 H CCS441-1
CS230-1K1a HCS15-3CS457-1K1a 4 6 7CS316-1CS542-1K1a 4 5 6 HCS442-3
CS231-1K1a 6CS17-1CS458-1K1a 4 7 HCS317-3CS543-1K1a 5 6 7 HCS443-3
CS232-1K1a 5CS12-7CS459-1K1a 4 7 CCS318-1CS544-1K1a 4 5 6 CCS445-1
CS233-1K1a 4CS11-2CS460-1K1a 4 C HCS320-1CS545-1K1a 4 5 6 7CS446-1
CS234-1K1a CCS14-2CS461-1K1a 4 5 6CS322-1CS546-1K1a 4 6 7 CCS447-1
CS367-1K1a 4 7CS203-1BCS462-1K1a 4 6 HCS323-3CS547-1K1a 4 5 7 HCS448-3
CS368-1K1a 4 HCS221-3CS463-1K1a 4 6 CCS324-1CS548-1K1a 4 5 7 CCS449-2
CS369-1K1a 4 5CS219-1CS464-1K1a 4 5 HCS326-1CS549-1K1a 5 6 7 CCS450-1
CS370-1K1a 4 6CS220-1CS465-1K1a 4 5 CCS327-1CS550-1K1a 5 7 C HCS452-2
CS375-1K1a 6 7CS205-1CS466-1K1a 5 6 7CS331-1CS551-1K1a 4 6 C HCS453-1
CS376-1K1a 6 HCS213-1CS467-1K1a 6 7 HCS332-3CS552-1K1a 4 5 C HCS454-1
CS377-1K1a 5 7CS204-1CS468-1K1a 6 C HCS334-1CS553-1K1a 6 7 C HCS455-1
CS378-1K1a 5 HCS215-3CS469-1K1a 5 6 HCS336-3CS554-1K1a 5 6 C HCS470-1
CS379-1K1a 7 HCS206-3CS470-1K1a 5 6 CCS337-1CS614-1K1a 4 5 6 7 HCS531-3
CS380-1K1a 4 CCS222-1CS471-1K1a 7 C HCS342-1CS615-1K1a 4 5 6 7 CCS533-1
CS381-1K1a 5 CCS216-2CS472-1K1a 6 7 CCS343-1CS616-1K1a 4 5 7 C HCS534-1
CS382-1K1a 6 CCS213-1CS473-1K1a 5 7 HCS345-3CS617-1K1a 4 6 7 C HCS535-1
CS383-1K1a 7 CCS207-2CS474-1K1a 5 7 CCS346-1CS618-1K1a 5 6 7 C HCS536-1
CS384-1K1a C HCS209-1CS475-1K1a 5 C HCS349-1CS619-1K1a 4 5 6 C HCS539-1
CS385-1K1a 5 6CS211-2CS540-1K1a 4 6 7 HCS440-3CS703-1K1a 4 5 6 7 C HCS612-1
  • a The parental strain from which individual and multiple PBP genes were deleted was E. coliCS109 (W1485 rpoS rph). Each mutant (“Strain” columns) was created by P1 transduction ofmrcA::res-npt-res from BMCS04-1K (CS109mrcA::res-npt-res) into the respective parental strain (“Parent” columns) and selection for kanamycin resistance. The parental strains are described by Denome et al (2). PBPs deleted from individual E. colistrains are abbreviated as follows (PBP, followed by gene name): 1a, = PBP 1a, mrcA; 4 = PBP 4, dacB; 5 = PBP 5, dacA; 6 = PBP 6, dacC; 7 = PBP 7,pbpG; C = AmpC, ampC; H = AmpH,ampH.