Table 2.

Oligonucleotides used in this study

DesignationSequencea
sseC-For2-165′-CTCGGATCCGGAAATCCCGCAGA-3′
sseC-Rev2-25′-GTACTGCAGATTAAGCGCGATAGC-3′
sseD-For35′-ATGAGATCTGAAGCGAGTAACGTAGCAC-3′
sseD-Rev2-85′-TGAAAGCTTACCTCGTTAATGCCC-3′
  • a Restriction sites introduced for cloning of PCR products are underlined.