Table 1.

Bacterial strains and plasmids used in this study

DesignationRelevant characteristicsReference or source
S. enterica serovar Typhimurium strains
 NCTC 12023Wild type, identical to ATCC 14028ATCCa
 P10E11 sscA::mTn5 29
 P9B7 ssaT::mTn5 29
 HH102 sseB::aphT, Kmr, nonpolar mutation11
 HH103HH102 containing psseB 11
 HH104 sseC::aphT, Kmr, nonpolar mutation11
 HH109 ssaV::aphT, Kmr, nonpolar mutation4
 MvP101 sseD::aphT, Kmr, nonpolar mutation20
 MvP131 ssaB::luc lacZ 3
E. colistrain
 M15pREP4Qiagen
Plasmids
 pEM25pMJH expressing SspH1-CyaA24
 pEM30pMJH expressing SspH2-CyaA24
 pQEHis tag expression vectors, Ampr Qiagen
 pJD14Codons 180 to 484 of sseC in pQE40This study
 pJD15Codons 2 to 195 of sseD in pQE30This study
 psseB sseB in pACYC184, Cmr 11
  • a ATCC, American Type Culture Collection.