Table 1.

Plasmids used in this studya

PlasmidRelevant characteristicOrigin
General
 pUC118Vector: Apr; multiple cloning site inlacZ Takara Shuzo Co.
 pUC119Vector: Apr; multiple cloning site in lacZ Takara Shuzo Co.
 pTrc99AExpression vector: Apr; multiple cloning site downstream of trc promoterAmersham Pharmacia Biotech
 pTrc6HispTrc99A-derived expression vector: Apr; C-terminal 6-His tagThis study
Plasmids carrying MF-type transporter ORFs
 pUCyajR2.0-kbKpnI-HindIII fragment containingyajR (putative transporter) gene cloned into pUC118, Apr This study
 pUCfsr1.8-kb EcoRI-SphI fragment containing fsr (fosmidomycin transporter) gene cloned into pUC118, Apr This study
 pUCmdfA1.6-kbKpnI-HindIII fragment containingmdfA (multidrug transporter) gene cloned into pUC118, Apr This study
 pUCyceE1.4-kb SphI-BamHI fragment containing yceE (putative transporter) gene cloned into pUC119, Apr This study
 pUCyceL1.7-kb SphI-BamHI fragment containing yceL (putative transporter) gene cloned into pUC119, Apr This study
 pUCydeA1.4-kb SphI-BamHI fragment containing ydeA (l-arabinose and IPTG transporter) gene cloned into pUC119, Apr This study
 pUCydeF1.7-kbSphI-BamHI fragment containing ydeF(putative transporter) gene cloned into pUC119, Apr This study
 pUCynfM2.5-kbSphI-BamHI fragment containing ynfL(putative regulator) and -M (putative transporter) genes cloned into pUC119, Apr This study
 pUCydhC2.4-kb SphI-BamHI fragment containing ydhB (putative regulator) and -C (putative transporter) genes cloned into pUC119, Apr This study
 pUCydiM1.7-kb SphI-BamHI fragment containing ydiM (putative transporter) gene cloned into pUC119, Apr This study
 pUCyebQ2.8-kb BamHI-EcoRI fragment containing kdgR (putative regulator) andyebQ (putative transporter) genes cloned into pUC119, Apr This study
 pUCbcr2.3-kb AccI-KpnI fragment containing yeiD (putative regulator) andbcr (bicyclomycin transporter) genes cloned into pUC119, Apr This study
 pUCemrKY7.5-kb SphI-BamHI fragment containing evgS/A (two-component system) andemrK/Y (putative MFP/putative transporter) genes cloned into pUC119, Apr This study
 pUCemrAB3.9-kb SalI-BamHI fragment containing emrR (regulator), -A (MFP), and -B (multidrug transporter) genes cloned into pUC119, Apr This study
 pUCemrD2.0-kbHindIII-SacI fragment containingemrD (multidrug transporter) gene cloned into pUC119, Apr This study
 pUCyidY2.7-kb PstI-BamHI fragment containing yidY (putative transporter) and -Z (putative regulator) genes cloned into pUC119, Apr This study
 pUCyieO2.2-kbPstI-BamHI fragment containing yieP(hypothetical protein) and -O (putative transporter) genes cloned into pUC119, Apr This study
 pUCyjiO2.8-kb SphI-BamHI fragment containing yjiO (putative transporter) and -N (hypothetical protein) genes cloned into pUC119, Apr This study
Plasmids carrying MATE-type transporter ORFs
 pUCydhE1.6-kbPstI-BamHI fragment containing ydhE(multidrug transporter) gene cloned into pUC119, Apr This study
Plasmids carrying RND-type transporter ORFs
 pUCacrAB6.0-kbPstI SmaI fragment containing acrR(regulator), -A (MFP), and -B (multidrug transporter) genes cloned into pUC119, Apr This study
 pUCcusA9.0-kbSphI-EcoRI fragment containing cusS(formerly ybcZ; sensor), cusR/C/F/B(ylcA/B/C/D: regulator/putative OMF/putative binding protein/putative MFP), and cusA (ybdE: putative metal ion or drug transporter) genes cloned into pUC119, Apr This study
 pUCyegMNOB14.2-kbSphI-EcoRI fragment containing yegK/L(putative two-component system), yegM/N/O/B (putative MFP/putative RND transporter/putative RND transporter/putative MF transporter), and baeS/R (putative two-component system) genes cloned into pUC119, Apr This study
 pUCacrD3.5-kbHindIII-SacI fragment containingacrD (drug transporter) gene cloned into pUC119, Apr This study
 pUCacrEF5.9-kbSphI-SalI fragment containing envR(regulator), acrE/F (MFP/multidrug transporter) genes cloned into pUC119, Apr This study
 pUCyhiUV4.7-kb PstI-BamHI fragment containing yhiU/V (putative MFP/putative transporter) genes cloned into pUC119, Apr This study
Plasmids carrying SMR-type transporter ORFs
 pUCemrE1.2-kbSmaI-BamHI fragment containing emrE(drug transporter) gene cloned into pUC118, Apr This study
 pUCydgFE1.1-kbSalI-BamHI fragment containing ydgF/E(putative transporter) genes cloned into pUC119, Apr This study
 pUCsugE1.0-kbPstI-SmaI fragment containing sugE(putative transporter) gene cloned into pUC119, Apr This study
Plasmids carrying ABC-type transporter ORFs
 pUCmdlAB4.2-kbSphI-EcoRI fragment containing mdlA/B(putative transporter) genes cloned into pUC119, Apr This study
 pUCybjYZ3.7-kbPstI-SalI fragment containing ybjY/Z(putative MFP/putative transporter) genes cloned into pUC119, Apr This study
 pUCyddA2.3-kbSalI-SacI fragment containing yddA(putative transporter) gene cloned into pUC119, Apr This study
 pUCyojIH4.0-kbSphI-SalI fragment containing yojI/H(putative transporter) genes cloned into pUC119, Apr This study
 pUCyhiH5.8-kbSphI-BamHI fragment containing yhiI/H(putative regulator/putative transporter) and yhhJ(hypothetical protein) genes cloned into pUC119, Apr This study
Plasmids carrying drug transporter ORFs under control of trcpromoter
 MF type
  pTrcHyajR1.4-kbNcoI-PstI fragment containing yajRgene cloned into pTrc6His, Apr This study
  pTrcHyceE1.2-kb NcoI-PstI fragment containing yceE gene cloned into pTrc6His, Apr This study
  pTrcHyceL1.2-kb NcoI-PstI fragment containing yceL gene cloned into pTrc6His, Apr This study
  pTrcHydeA1.2-kb NcoI-PstI fragment containing ydeA gene cloned into pTrc6His, Apr This study
  pTrcHydeF1.2-kb EcoRI-PstI fragment containing ydeF gene cloned into pTrc6His, Apr This study
  pTrcHynfM1.3-kb NcoI-PstI fragment containing ynfM gene cloned into pTrc6His, Apr This study
  pTrcHydhC1.2-kb NcoI-SalI fragment containing ydhC gene cloned into pTrc6His, Apr This study
  pTrcHydiM1.2-kb NcoI-SalI fragment containing ydiM gene cloned into pTrc6His, Apr This study
  pTrcHyebQ1.5-kb NcoI-SalI fragment containing yebQ gene cloned into pTrc6His, Apr This study
  pTrcHyegB1.4-kb NcoI-PstI fragment containing yegB gene cloned into pTrc6His, Apr This study
  pTrcHbcr1.2-kb NcoI-PstI fragment containing bcr gene cloned into pTrc6His, Apr This study
  pTrcHyidY1.2-kbNcoI-PstI fragment containing yidYgene cloned into pTrc6His, Apr This study
  pTrcHyieO1.4-kb NcoI-PstI fragment containing yieO gene cloned into pTrc6His, Apr This study
  pTrcHyjiO1.2-kb NcoI-PstI fragment containing yjiO gene cloned into pTrc6His, Apr This study
 RND type
  pTrcHcusAB4.4-kbNcoI-XbaI fragment containing cusA andcusB genes cloned into pTrc6His, Apr This study
  pTrcHyegN3.1-kbNcoI-PstI fragment containing yegNgene cloned into pTrc6His, Apr This study
  pTrcHyegO3.1-kb EcoRI-PstI fragment containing yegO gene cloned into pTrc6His, Apr This study
  pTrcHacrD3.1-kb NcoI-SalI fragment containing acrD gene cloned into pTrc6His, Apr This study
  pTrcHacrEF4.3-kb SacI-PstI fragment containing acrE and acrF genes cloned into pTrc6His, Apr This study
 SMR type
  pTrcHydgFE0.7-kbEcoRI-PstI fragment containing ydgFand ydgE genes cloned into pTrc6His, Apr This study
  pTrcHsugE0.5-kbNcoI-PstI fragment containing sugEgene cloned into pTrc6His, Apr This study
 ABC type
  pTrcHmdlA1.8-kbEcoRI-PstI fragment containing mdlAgene cloned into pTrc6His, Apr This study
  pTrcHmdlB1.8-kb NcoI-PstI fragment containing mdlB gene cloned into pTrc6His, Apr This study
  pTrcHybjZ2.0-kb NcoI-PstI fragment containing ybjZ gene cloned into pTrc6His, Apr This study
  pTrcHyddA1.7-kb NcoI-PstI fragment containing yddA gene cloned into pTrc6His, Apr This study
  pTrcHyojI1.6-kb NcoI-PstI fragment containing yojI gene cloned into pTrc6His, Apr This study
  pTrcHyojH1.7-kb EcoRI-PstI fragment containing yojH gene cloned into pTrc6His, Apr This study
  pTrcHyhiH2.7-kb NcoI-PstI fragment containing yhiH gene cloned into pTrc6His, Apr This study
  • a DNA fragments cloned in pUC vectors contained the endogenous promoter regions that were expected to work at the drug resistance measurements without induction.