Table 2.

Properties of primers used in this study

PrimerSequence (5′–3′) and salient featuresAnnealing position
TadA UP Embedded Image 5′ end of CU1000N tadA
TadA DWN3′ end of CU1000NtadA
TadA-T7 DWN3′ end of CU1000NtadA
TadA K210A-1Overlapping K210 codon and directed toward tadA 5′
TadA K210A-2Overlapping K210 codon and directed toward tadA 3′
TadA K210Q-1Overlapping K210 codon and directed toward tadA 5′
TadA K210Q-2Overlapping K210 codon and directed toward tadA 3′