Table 3.

Structural parameters of forespore PG produced by pbpF and pbpG mutant strainsa

GenotypeTime in sporulation (h)% Spore PG madeb% Muramic acid with:
Side chain ofc:Cross-linked side chain
Lactaml-AlaTriPTP
pbpF 3.524.67.972.115.414.8
44.530.333.327.58.96.3
4.513.541.444.29.54.92.7
522.543.538.67.710.22.6
5.53344.530.95.219.32.9
646.545.629.33.721.33.0
6.559.545.526.53.324.73.2
77546.126.62.724.63.0
7.58946.424.82.426.43.1
810046.522.82.328.43.4
2410048.521.61.528.43.4
pbpG 3.59.513.217.156.812.911.1
418.532.434.326.27.25.5
4.527.537.335.617.79.44.1
537.539.829.712.018.54.0
5.55241.027.49.522.03.8
67142.726.07.024.23.9
6.578.543.424.76.125.73.8
78644.424.65.425.63.7
7.593.545.123.64.926.43.9
810046.123.74.226.03.6
2410047.718.13.231.04.1
pbpF pbpG 3.550.00.080.919.114.0
41110.911.553.424.212.1
4.517.518.814.929.627.79.7
53616.77.321.055.07.2
5.55714.35.411.169.36.5
67015.65.68.270.66.4
6.58116.45.57.270.96.5
789.517.75.76.670.06.3
7.59518.76.16.169.26.1
810019.36.05.868.96.0
240
  • a Forespore PG was purified from culture samples taken every 15 min between the fourth and seventh hours of sporulation, but only data for samples taken every 30 min are shown.

  • b These values are derived from the interpolation of culture muramic acid contents as determined by amino acid analyses (28).

  • c Lactam, muramic δ-lactam;l-Ala, single alanine; TriP, tripeptide; TP, tetrapeptide.