Table 1.

Bacterial strains and plasmids used in this study

Straina or plasmidRelevant propertiesbSource or reference
Escherichia coli
  DH5α recA1 gyrA (Nal), Δ(lacIZYA-argF) [Φ80dlacΔ(lacZ)M15]Gibco-BRL
  HB101F hsd-20 recA13 ara-14 proA2 lacY1 galK2 rpsL20 (Str) xyl-5 mtl-1 supE44 3
Streptococcus agalactiae
  NEM316Serotype III isolated from neonate blood culture (early onset disease)16
  NEM1636NEM316 dltAΩaphA-3 Km DltA This work
  NEM1637NEM316dltRΩaphA-3 Km DltR This work
  NEM1638NEM1636::pAT113/SpΩdltA Km, Sp DltA/DltA+ This work
  NEM1639NEM316 dltAΩaphA-3 dltRΩcat Cm Km DltRDltA This work
  NEM1687NEM1637::pAT113/SpΩdltR Km Sp DltR/DltR+ This work
  NEM1693NEM316 dltSΩaphA-3 Km DltS This work
  NEM1786NEM316dltAΩaphA-3 dltSΩaad6-3 Km Sm DltS DltA This work
Enterococcus faecalis BM4110Streptomycin-resistant derivative of JH2; Str8
Listeria monocytogenesLO28-StrStreptomycin-resistant derivative of LO28; Str4
 pRK24Ap Tc Tra+Mob+ (IncP)38
 pTCV-lac Em Km Mob+ (IncP); oriR pACYC184; oriRpAMβ1; MCS; promoterless spoVG-lacZ translational fusion29
 pG+host5Em; ColE1 replicon, thermosensitive derivative of pGK12; MCS pBluescript1
 pAT113/SpSp Mob+ (IncP); oriRpACYC184; attTn1545, lacZa +; MCS pUC195
 pTCV-erm Em Km Mob+ (IncP);oriR pACYC184; oriR pAMβ1; MCSlacZa + Poyart and Trieu-Cuot, unpublished data
 pTCV-int Em Km Mob+ (IncP); pTCV-erm derivative with 1.8-kbEcoRI-PstI fragment carrying PdltRΩintTn1545 This work
  • a Bacteria were grown in BHI broth or agar.

  • b Ap, Cm, Em, Km, Nal, Sp, Str (or Sm), and Tc represent resistance to ampicillin, chloramphenicol, erythromycin, kanamycin, nalidixic acid, spectinomycin, streptomycin, and tetracycline, respectively. attTn and MCS, transposon attachment site and multiple cloning site, respectively.