Table 3.

Phenotypes of the virB11.i4 alleles

PC1011(pJC8xxx) or control straini4 insertion siteaDominancebT-DNA transfercTransfer by mixed infectioncpML122ΔKm mobilization (Tcs/input donor)c,dT-pilus productiona
VirE2T-strand/D2
pJC8018L18+<10−7
pJC8056D56<10−7
pJC8075L75+++9.5 × 10−6
pJC8088L885 × 10−7
pJC8115E1152 × 10−7
pJC8135Q135+++NANA2 × 10−3 +++
pJC8168C168++1.5 × 10−5
pJC8196E196+++<10−7
pJC8217G217+++NANA5.5 × 10−4 +++
pJC8237P237+++NANA1.2 × 10−3 +++
pJC8260G2601 × 10−7
pJC8281F2811.3 × 10−7
pJC8302L302++9.3 × 10−6
pJC8323E323+1.5 × 10−7
A348WT+++NANA3 × 10−3 +++
PC1011ΔvirB11 <10−7
PC1011(pXZB100)(PvirB::virB11)+++NANA3 × 10−3 +++
  • a Indicated residue immediately precedes i4 insertion.

  • b Virulence of thevirB11/virB11.i4 merodiploid strain is strongly (+++) or weakly (+) attenuated or not attenuated (−).

  • c See footnotes to Table 1 for meaning of symbols.

  • d Data are the means of three trials from a single experiments. Three independent experiments yielded similar results.