Table 1.

Mutants defective in paralytic killing

StrainInsertion siteaGenebFunction% Killingc
PAO199 (±0)
Class I (strongly avirulent strains)
 MP5033,571,648eda2-Keto-3-deoxy-6-phosphogluconate aldolase; Entner-Doudoroff pathway4 (±2)
 MP505435,468proCΔ-1-Pyrroline-5-carboxylate reductase; proline biosynthesis4 (±3)
 MP506435,230proCΔ-1-Pyrroline-5-carboxylate reductase; proline biosynthesis0 (±0)
 MP504812,969PA0745dProbably enoyl-coenzyme A hydratase; fatty acid degradation13 (±10)
 MP5072,415,450hcnCHydrogen cyanide synthase3 (±2)
 MP50845,846PA0041dHomologue offhaB, Bordetella filamentous hemagglutinin0 (±0)
 MP5014,423,808PA3946dHomologue of bvgS, Bordetella two-component sensor kinase virulence gene regulator0 (±0)
 MP5021,015,249gacSHomologue ofPseudomonas syringae two-component sensor kinase controlling disease lesion formation9 (±5)
 MP511Unsequencede0 (±0)
Class II (moderately avirulent strains)
 MP5543,572,897zwfGlucose-6-phosphate dehydrogenase; Entner-Doudoroff pathway41 (±19)
 MP5556,098,814soxASarcosine oxidase38 (±20)
 MP5566,100,002soxASarcosine oxidase39 (±19)
 MP5571,032,886purMPhosphoribosylaminoimldazole synthetase; purine biosynthesis92 (±3)
 MP5584,216,505purLPhosphoribosylformylglycinamidine synthase; purine biosynthesis90 (±5)
 MP559874,405prpBCarboxyphosphonoenolpyruvate phosphonomutase; fatty acid and phospholipid metabolism83 (±15)
 MP560873,168prpCCitrate synthase 272 (±28)
 MP5611,758,910gpdAGlycerol-3-phosphate dehydrogenase; specific to fatty acid and phospholipid metabolism57 (±23)
 MP5622,927,500PA2587Putative salicylate hydroxylase; quinolone signal synthesis27 (±16)
 MP5716,193,910znuBPermease of ABC zinc transporter39 (±20)
 MP5734,290,026phpAAminopeptidase; protein modification, alginate biosynthesis28 (±13)
 MP5745,993,742algCLipopolysaccharide and alginate biosynthesis75 (±15)
 MP5725,100,129pilWType 4 pili56 (±10)
 MP5511,086,674PA1003dPutative transcriptional regulator with LysR family signature31 (±22)
 MP5525,304,505PA4725dPutative amino acid permease fused to putative two-component sensor histidine kinase42 (±15)
 MP5535,304,930PA4725dPutative amino acid permease fused to putative two-component sensor histidine kinase76 (±16)
  • a The transposon insertion site corresponds to the chromosomal location in the PAO1 single contig sequence ( ).

  • b Boldface type indicates known P. aeruginosa genes; lightface type indicates close homologues of known genes. PA numbers are designations assigned by the web site ( ).

  • c Percentages of killing are averages based on at least three independent killing assays for each strain. The numbers in parentheses are standard errors of the means.

  • d Gene not experimentally characterized in studies of pseudomonads.

  • e Repeated attempts to sequence were unsuccessful.