Table 3.

Activities of PdltR and PdltA in S. agalactiae NEM316 and derivatives

Promoter fused tospoVG-lacZa β-Galactosidase activity (mean arbitrary units ± SD)b
NEM316NEM1636 (DltA)NEM1638 (DltA/DltA+)NEM1637 (DltR)NEM1639 (DltRDltA)NEM1687 (DltR/DltR+)NEM1693 (DltS)NEM1786 (DltS DltA)
None<1<1<1<1<1<1<1<1
PdltR(LA11-LA12)201 ± 19504 ± 14314 ± 12121 ± 9107 ± 10390 ± 19134 ± 10147 ± 12
PdltA(LA14-LA17)34 ± 4100 ± 872 ± 540 ± 872 ± 7NTc NTNT
  • a DNA fragments containing promoter activity were amplified with the primers indicated in parentheses and cloned into pTCV-lac.

  • b β-Galactosidase was assayed in cells collected in mid-exponential phase. Experiments were performed in triplicate, and the mean values ± 1 standard deviation (SD) are indicated.

  • c NT, not tested.