Table 2.

Activities of PdltR and PdltA in various gram-positive bacteria

Promoter fused tospoVG-lacZa β-Galactosidase activity (mean arbitrary units ± SD)b
S. agalactiae NEM316E. faecalisBM4110L. monocytogenes LO28-Sm
None<1<1<1
PdltR(LA11-LA12)201 ± 19101 ± 3.558 ± 1.5
PdltA(LA14-LA17)34 ± 4<1<1
PaphA-3 10.5 ± 0.979.3 ± 6.355.5 ± 3.1
  • a DNA fragments containing promoter activity were amplified with the primers indicated in parentheses and cloned into pTCV-lac. The activities of PaphA-3 were from reference 29.

  • b β-Galactosidase was assayed in cells collected in mid-exponential phase. Experiments were performed in triplicate, and the mean values ± 1 standard deviation (SD) are indicated.