Table 1.

Bacterial strains

StrainaGenotypebPedigreecReference(s) and/or derivationd
BW37IN(rrnD-rrnE)I tna bglR::ISe trpR ilv rpsL rph-1 W3110 via BW33 38, 40
BW5328Δ(lacIZYA argF)U169 rph-1 rpoS396(Am)recA1 robA1 creC510 hsdR514 BD792 via BW5206 37
BW23473 Δ(lacIZYA argF)U169 rph-1 rpoS396(Am) robA1 creC510 hsdR514 ΔendA9 uidAMluI)::pir(wt)recA1 BD792 via BW23438 15, 16
BW23838 lacI q rrnB3ΔlacZ4787 ΔphoBR580 ΔcreABCD154 hsdR514 Δ(pta ackA hisQ hisP)TA3516 phn(EcoB) DE(araBAD)567DE(rhaBAD)568 rph-1 rpoS396(Am)uidAMluI)::pir(wt) ΔendA9 recD1014 recA1) BD792 via BW23832Srl+ with P1kc on BW8078; 14
BW24249 lacI q rrnB3ΔlacZ4787 ΔphoBR580 ΔcreABCD154 hsdR514 Δ(pta ackA hisQ hisP)TA3516 phn(EcoB) DE(araBAD)567DE(rhaBAD)568 rpoS396(Am)rph-1ΔendA9 galU95 uidAMluI)::pir(wt)recA1 BD792 via BW24217 Srl+ with P1kc on BW8078
BW24304 lacI q rrnB3 ΔlacZ4787 ΔphoBR580ΔcreABCD154 hsdR514 Δ(pta ackA hisQ hisP)TA3516 phn(EcoB) DE(araBAD)567 DE(rhaBAD)568 rph-1 rpoS396(Am) ΔendA9 galU95 uidAMluI)::pir-116 recA1 BD792 via BW24296 Srl+ with P1kc on BW8078
BW24320 lacI q rrnB3ΔlacZ4787 ΔphoBR580 ΔcreABCD154DE(araBAD)567 DE(rhaBAD)568 rph-1 BD792 via BW24310 Cys+ with P1kc on MG1655; 24
BW25113 lacI q rrnB3 ΔlacZ4787 hsdR514 DE(araBAD)567DE(rhaBAD)568 rph-1 BD792 via BW25083Pro+ with P1kc on BW24321; 10, 24
BW25141 lacI q rrnB3ΔlacZ4787 hsdR514 DE(araBAD)567DE(rhaBAD)568 ΔphoBR580 rph-1 galU95ΔendA9 uidA(ΔMluI)::pir(wt)recA1 BD792 via BW25140 Srl+ with P1kc on BW8078; 10, 24
BW25142 lacI q rrnB3 ΔlacZ4787 hsdR514 DE(araBAD)567DE(rhaBAD)568 ΔphoBR580 rph-1 galU95ΔendA9 uidA(ΔMluI)::pir-116 recA1 BD792 via BW25137 Srl+ with P1kc on BW8078
BW25695 lacI q rrnB3 ΔlacZ4787 hsdR514 att P22(EcoB) DE(araBAD)567DE(rhaBAD)568 rph-1 BD792 via BW25367Pro+ with P1kc on BW25676
  • a All strains are λ and F.

  • b All known mutations are indicated.

  • c The ancestral strain from another lab and its immediate ancestor in our lab are given.

  • d Additional information is given in Materials and Methods.

  • e IS, insertion sequence.