Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5αphe | DH5αphe::Tn10dCm | 4 |
| HB101 | lacY1 galK2 supE44 ara-14 proA2 rpsL20 recA13 xyl-5 mtl-1 hsdS20 mcrB mrr | 12 |
| S17-1 | C600::RP-4 2-(Tc::Mu)(Km::Tn7) thi pro hsdR hsdM + recA | 25 |
| R. sphaeroides | ||
| 2.4.1 | Wild type | W. R. Sistrom |
| TSPO1 | tspO::Kmr | 31 |
| APP11 | appA::Tpr | 6 |
| APP-TSPO | APP11tspO::Kmr | This study |
| PUC-ZWT | lacZY::Ω Smr/Spr A′ inserted at theXmnI sites within pucB of the wild type; B800-850− | 13 |
| PUCB-TSPO | PUC-ZWTtspO::Kmr | This study |
| Plasmids | ||
| pRK415 | Tcr | 10 |
| pBSIIKS+ | Apr; with T3 and T7 promoters | Stratagene |
| pUC4K | Source of Kmr | Pharmacia |
| pSUP202 | pBR325 derivative, Mob+ Ap+ Cm+ Tc+ | 25 |
| pAS204 | pRK415 containing the 2.1-kb SstIcrtB, tspO fragment from pUI8487; Tcr | A. Suwanto |
| pUI1110 | pSUP202 containing 3.7-kbtspO::Km fragment inserted at the SspI site; Tc+ Km+ | M. Wood and S. Kaplan |
| pUI1124 | pBSIIKS+ containing tspO under PrrnB Ap+ | M. Wood and S. Kaplan |
| pUI1830Tp | Smr/Spr Tp+ puf::lacZ | J. I. Oh and S. Kaplan |
| pCF200Km | Smr/SprKm+ puc::lacZ | 13 |
| pUI2701 | Derivative of pRK415 harboring 1.1-kb KpnI fragment of pUI1124 containing tspO under PrrnB; Tcr | 31 |
| pUI2730 | Tcr; derivative of pRK415 containing hemN | 30 |
| pUI2732 | Tcr; derivative of pRK415 containinghemN and tspO under PrrnB | 30 |