Table 3.

Crt and Bchl accumulated by wild-type and mutant strains of R. sphaeroides

StrainAmt (μg/mg of protein) for growth condition:
AerobicaSemiaerobicbDark-DMSOc
Bch1CrtBch1CrtBch1Crt
2.4.1 (wild type)0.061 ± 0.0050.026 ± 0.0021.96 ± 0.220.19 ± 0.021.71 ± 0.210.27 ± 0.03
TSPO10.076 ± 0.0080.034 ± 0.0032.43 ± 0.270.25 ± 0.032.34 ± 0.250.32 ± 0.04
TSPO1(pUI2701)0.060 ± 0.0090.027 ± 0.0021.38 ± 0.190.16 ± 0.021.31 ± 0.180.22 ± 0.02
APP110.004 ± 0.0030.003 ± 0.0020.09 ± 0.050.08 ± 0.030.06 ± 0.040.04 ± 0.02
APP-TSPO0.004 ± 0.0020.002 ± 0.0020.05 ± 0.040.06 ± 0.020.07 ± 0.050.05 ± 0.03
APP-TSPO(pUI2701)0.005 ± 0.0020.003 ± 0.0020.06 ± 0.040.06 ± 0.020.06 ± 0.030.03 ± 0.02
PUC-ZWT0.078 ± 0.0070.031 ± 0.0030.60 ± 0.090.18 ± 0.020.66 ± 0.080.22 ± 0.02
PUCB-TSPO0.079 ± 0.0080.030 ± 0.0030.59 ± 0.070.23 ± 0.030.59 ± 0.150.27 ± 0.03
PUCB-TSPO(pUI2701)0.075 ± 0.0070.037 ± 0.0040.27 ± 0.060.14 ± 0.020.25 ± 0.050.15 ± 0.03
  • a Strains were grown by sparging with 69% N2–30% O2–1% CO2 to a cell density of approximately 1.8 × 108 cells/ml.

  • b Strains were grown by sparging with 97% N2–2% O2–1% CO2 to a cell density of approximately 1.8 × 108 cells/ml.

  • c Strains were grown anaerobically in the dark with DMSO (0.5% [vol/vol]) and yeast extract (0.1% [vol/vol]) to a cell density of approximately 1.8 × 108 cells/ml.