Table 1.

Strains and plasmids used in this study.

Strain or plasmid Description Reference or source
Strains
 PB114 (λDB173)ΔminλPlac::minCD 11
 JKD7.2 ftsZ::kan recA56; FtsZ supplied by pKD3c 10
 JKD7.2 (λDB173) λDB173 lysogen of JKD7.2This study
 PS269 PB114 (λDB173/pSEB103) This study
 PS270 PB114 (λDB173/pSEB104) This study
 PS294JKD7.2 (λDB173/pBEF0) This study
 PS293 JKD7.2 (λDB173/pBEF2) This study
 PS309 JKD7.2 (λDB173/pBEF9)This study
 PS310 JKD7.2 (λDB173/pBEF100) This study
 PS311 JKD7.2 (λDB173/pBEF114) This study
 PS299PB114 (λDB173/pJPB209) This study
 PS300 PB114 (λDB173/pJPB222) This study
 PS301 PB114 (λDB173/pJPB223) This study
 PS302 PB114 (λDB173/pSEB25) This study
 PS298 PB114 (λDB173/pSEB162) This study
Plasmids
 pBEF0 Spcr; wild-type ftsZ on a pGB2 derivative 4
 pBEF2 Spcr; pBEF0 but contains ftsZ2 4
 pBEF9 Spcr; pBEF0 but carries ftsZ9 4
 pBEF100 Spcr; pBEF0 but carries ftsZ101 and ftsZ114 mutations4
 pBEF114 Spcr; pBEF0 but carriesftsZ114 mutation (same as ftsZ103, sfiB103, orsfiB114) 4
 pKD3c Cmr; temperature-sensitive pSC101 derivative containing ftsAZ andenvA 2
 pSEB12 Cmr; mini-F plasmid containing minCDE under the control of their own promoters26
 pSEB14 Cmr; mini-F plasmid encodingminCD under the control of their own promoters 26
 pJPB222 Spcr; same as pJPB223 butftsQAZ genes are in opposite orientation to theaadA gene; the level of expression of ftsQAZis lower than in pJPB223 Gift from Jean-Pierre Bouché
 pJPB223 Spcr; ftsQAZ cloned on pJPB209, aadA, and ftsQAZ are in the same orientation 26
 PSEB25 Spcr; pJPB223 with thePvuII fragment deleted, yielding an internal in-phase deletion of ftsA Gift from Jean-Pierre Bouché
 pSEB162 Spcr; pJPB223 with the EcoRI fragment containing ftsZ deleted This study
 pSEB103 Spcr; zipA-GFP under the control of PBAD on a pGB2 derivative This study
 pSEB104 Spcr; ftsZ-GFP under the control of PBAD on a pGB2 derivative This study
 pJPB209 Spcr; cloning vector derived from pGB2 26
 pGB2 Spcr; cloning vector with the pSC101 origin of replication 9