Table 2.

RsmA-dependent production of P. aeruginosaautoinducers 3-oxo-C12-HSL and C4-HSLa

Strain (genotype)Concn (μM) at indicated OD600
3-oxo-C12-HSLC4-HSL
0.62.50.62.5
PAO1 (rsmA +)0.18 ± 0.020.53 ± 0.090.25 ± 0.16.0 ± 1.0
PAZH13 (rsmA)0.45 ± 0.010.47 ± 0.050.97 ± 0.1413.0 ± 1.0
PAO1/pME3849 (rsmA ++)0.08 ± 0.060.13 ± 0.030.06 ± 0.022.0 ± 0
  • a Concentrations of 3-oxo-C12-HSL and C4-HSL were estimated for P. aeruginosa strains grown in NYB by TLC analysis (see Materials and Methods) when cells reached an OD600 of 0.6 and 2.5. The experiment was performed in triplicate. Values are means ± standard deviations.