Table 2.

Identification and function of genes which are differentially expressed in P. putida 6 h after initial attachment to the inner surface of tubinga

Class and functionGeneExpressionLocus
Class I
 Carbon catabolism/amino acid metabolism cofactor metabolism
  Two-component responsegltRPA3192
  RibokinaserbsKPA1950
  Probable asparagine synthetaseasnBPA2084
  Probable acyl-coenzyme A dehydrogenaseNIPA2015
  Probable aldehyde dehydrogenaseadhAPA2217
  Leucyl-tRNA synthaseleuSPA3987
  Thiamine phosphate pyrophosphorylase, thiaminethiEPA3976
  Hypothetical protein, ubiquinone biosynthesis proteinaarFPA5065
Class II
 Membrane proteins/transport
  Outer membrane lipoproteinnlpD+PA3623
  ABC transporterpotB+PA3608
  Resistance/nodulation/cell division multidrug efflux pumpmex+PA0425
  Probable K+ efflux transporterybaL+PA5518
  General secretion pathway protein FxcpS+PA3102
Class III
 Polysaccharides/lipopolysaccharide biosynthesis
  Negative regulator for alginate biosynthesismucC+PA0765
  Putative capsule polysaccharide export protein precursor (Klebsiella pneumoniae partial YC04 gene)NI+NI
  UDP-3-O-[hydroxylauroyl] glucosamineN-acyltransferaselpxD+PA3646
  Lipopolysaccharide biosynthesis genewbpG+PA3150
Class IV
 Motility
  Flagellar synthesis regulatorfleNPA1454
  Flagellar basal body rod proteinflgGPA1082
  Na+-translocating NADH:ubiquinone oxidoreductase Nrq2nqrBPA2998
  Two-component response regulatorpilR+PA4547
  Type IV fimbrial biosynthesis genepilC+PA4527
  Chemotactic methyltransferase CheR homologpilK+PA0412
DNA replication and rRNA maturation
 Exoribonuclease V beta chainrecBPA4284
 rRNA (adenine N6, N6)-dimethyltransferaseksgAPA0592
Antibiotic resistance/virulence factors
 ChitinasechiC+PA2300
 β-LactamaseampC+PA4410
 Streptomycin 3′-phosphotransferasestr+PA1858
  • a Subtracted cDNA libraries were generated using subtractive hybridization and the resulting DNA sequences representing differentially expressed genes were identified by sequence comparison. The identification was carried out using the BLASTN and BLASTX program (1). The gene names correspond to the gene names given in the P. aeruginosa Genome Project website. NI, not identified. −, down-regulated in attachedP. putida cells; +, up-regulated in attachedP. putida cells.