Table 2.

Identification and function of genes which are differentially expressed in P. putida 6 h after initial attachment to the inner surface of tubinga

Class and functionGeneExpressionLocus
Class I
 Carbon catabolism/amino acid metabolism cofactor metabolism
  Two-component response gltR PA3192
  Ribokinase rbsK PA1950
  Probable asparagine synthetase asnB PA2084
  Probable acyl-coenzyme A dehydrogenaseNIPA2015
  Probable aldehyde dehydrogenase adhA PA2217
  Leucyl-tRNA synthase leuS PA3987
  Thiamine phosphate pyrophosphorylase, thiamine thiE PA3976
  Hypothetical protein, ubiquinone biosynthesis protein aarF PA5065
Class II
 Membrane proteins/transport
  Outer membrane lipoprotein nlpD +PA3623
  ABC transporter potB +PA3608
  Resistance/nodulation/cell division multidrug efflux pump mex +PA0425
  Probable K+ efflux transporter ybaL +PA5518
  General secretion pathway protein F xcpS +PA3102
Class III
 Polysaccharides/lipopolysaccharide biosynthesis
  Negative regulator for alginate biosynthesis mucC +PA0765
  Putative capsule polysaccharide export protein precursor (Klebsiella pneumoniae partial YC04 gene)NI+NI
  UDP-3-O-[hydroxylauroyl] glucosamineN-acyltransferase lpxD +PA3646
  Lipopolysaccharide biosynthesis gene wbpG +PA3150
Class IV
  Flagellar synthesis regulator fleN PA1454
  Flagellar basal body rod protein flgG PA1082
  Na+-translocating NADH:ubiquinone oxidoreductase Nrq2 nqrB PA2998
  Two-component response regulator pilR +PA4547
  Type IV fimbrial biosynthesis gene pilC +PA4527
  Chemotactic methyltransferase CheR homolog pilK +PA0412
DNA replication and rRNA maturation
 Exoribonuclease V beta chain recB PA4284
 rRNA (adenine N6, N6)-dimethyltransferase ksgA PA0592
Antibiotic resistance/virulence factors
 Chitinase chiC +PA2300
 β-Lactamase ampC +PA4410
 Streptomycin 3′-phosphotransferase str +PA1858
  • a Subtracted cDNA libraries were generated using subtractive hybridization and the resulting DNA sequences representing differentially expressed genes were identified by sequence comparison. The identification was carried out using the BLASTN and BLASTX program (1). The gene names correspond to the gene names given in the P. aeruginosa Genome Project website. NI, not identified. −, down-regulated in attachedP. putida cells; +, up-regulated in attachedP. putida cells.