Table 1.

Bacterial strains and plasmid used in the study

Strain or plasmidRelevant characteristicsaSource or reference
S. mutans
BM71Wild type, Ems, KmsG. H. Bowden, University of Manitoba
SMCC-L1BM71::pComC-KO, ComC, Emr, KmsThis study
SMCD-L1BM71::pComD-KO, ComD, Emr, KmsThis study
SMCE-L1BM71::pComD-KO, ComE, Emr, KmsThis study
Plasmid, pDL289bE. coli/Streptococcusshuttle vector, KmrD. J. LeBlanc, Indiana University
  • a Em, erythromycin; Km, kanamycin.

  • b pDL289 was used as donor DNA to confirm competence phenotype of all the mutants.