Table 1.

Bacterial strains and plasmids

Strain or plasmidRelevant genotype, phenotype, or roleaSource or reference
P. aeruginosa
 PAK N1 rpoN::Genr S. Lory (28)
 PA14Wild typeM. Schroth (52)
 PA14rpoN::Genr Contains Genr cassette inserted into rpoN This study
 PA14(pSMC21)PA14 expressing GFP56
 PA14rpoN::Genr(pSMC21) rpoN::Genrexpressing GFPThis study
E. coli
 DH5aF lacZΔM15endA1 recA1 hsdR17 supE44 thi-1 gyrA relA1λ; host for cosmid library and other plasmidsBethesda Research Laboratories (20)
 MM294(pRK2013)Donor of transfer functions for triparental crosses14
Plasmids
 pJSR1Apr, cosmid cloning vector52
 pBSK(+)Apr, cloning vectorStratagene, Inc.
 pBR322AprTetr, cloning vectorNew England Biolabs, Inc.
 pKI11Source of P. aeruginosa PAK rpoNgeneS. Lory (28)
 pPAR44-kb fragment containing PA14rpoN gene in pBSK(+)This study
 pPAR4SR4-kb fragment containing PA14 rpoN gene in pJSR1This study
 pRPONgentPA14 rpoN::Genr in pBR322This study
 pRPON10PA14 rpoN gene in pJSR1This study
 pSMC21Derivative of plasmid pSMC2; carries the A. victoria GFPG. A. O'Toole (7)
  • a Apr, ampicillin resistance; Tetr, tetracycline resistance.