Table 4.

SarA-upregulated genes

ORFaGenebPatterncFunctiondN315 ORFeRole categoryfSar boxg
3298 capJ 1 (2)Capsule geneSA0153Adaptation
1321 aroC 1 (1)Chorismate synthetaseSA1299Amino acid metabolism
997 hutG 1 (1)ArginaseSA2125Amino acid metabolismYes
452 metK 1 (2) S-Adenosylmethionine synthetaseSA1608Amino acid metabolism
2628 pepF 1 (4)PeptidaseSA1216Amino acid metabolism
7 sdhB 1 (2) l-Serine dehydrataseSA2319Amino acid metabolism
8211 (2)AcetyltransferaseSA1931Amino acid metabolismYes
1765 epiF 1 (2)Epidermin-like biosynthetic clusterAntibiotic production
4746 epiP 1 (2)Serine proteaseAntibiotic production
44421 (2)Bacteriophage proteinSA1786Bacteriophage related
36881 (7)Predicted membrane proteinSA2436Bacteriophage related
1973 rodA 1 (3)Cell division membrane proteinSA1888Cell division
184 femA 1 (2) mecresistanceSA1206Cell wall
2497 tagF 1 (2)Glycosyl/glycerophosphate transferasesSA0244Cell wall
3495 uppS 1 (4)Undecaprenyl diphosphate synthaseSA1103Cell wall
6001 (3)Putative glycosyltransferasesSA0523Cell wall
47571 (2)6-Pyruvoyl-tetrahydropterin synthaseSA0666Coenzyme metabolism
3095 dnaG 1 (2)DNA primaseSA1391DNA replication
3733BH23911 (1)Short-chain alcohol dehydrogenasesSA1123Lipid metabolism
3898 crtN 1 (2)Putative phytoene dehydrogenaseSA2351Lipid metabolism
797 purA 1 (3)Adenylosuccinate synthaseSA0016Nucleic acid metabolism
373 pyrAA 1 (1)Carbamoyl-phosphate synthetaseSA1045Nucleic acid metabolism
783 rpmH 1 (1)Ribosomal protein L34SAS093Ribosomal proteins
1118 queA 2 (4) S-AdenosylmethionineSA1466RNA modification
2444 agrA 1 (1) agrresponseSA1844Signal transductionUpstream
1426 agrB 1 (1) agrresponseSA1842Signal transductionYes
2443 agrC 2 (2) agrresponseSA1843Signal transductionUpstream
1425 agrD 1 (1) agrresponseSAS066Signal transductionUpstream
2944 phoP 1 (1)Response regulatorSA1516Transcriptional regulationYes
52591 (3)Transcriptional regulator, GntR familySA0434Transcriptional regulation
28521 (2)Putative transcriptional regulatorSA1120Transcriptional regulation
1545 prfA 1 (1)Protein chain release factor ASA1920Translation termination
4309 fhuD 1 (2)Ferrichrome-binding periplasmic proteinSA2079Transport
614 gltS 1 (1)Glutamate permeaseSA2135Transport
2601 opp-2B 1 (4)Putative oligopeptide transporterSA1214TransportYes
2711 (3)Putative permeaseSA0099Transport
2721 (2)Putative transportSA0100TransportUpstream
46021 (2)Bmr-like proteinSA2241Transport
42001 (2)SA0085Unknown
9921 (3)Conserved hypotheticalSA0174Unknown
2431 (1)Conserved hypotheticalSA0350Unknown
20951 (1)Hypothetical proteinSA0453UnknownYes
26341 (3)UnknownSA0754Unknown
43701 (2)SA1037Unknown
8901 (2)Conserved hypotheticalSA1240Unknown
24791 (2)Conserved hypotheticalSA1275Unknown
27821 (2)Hypothetical proteinSA1319Unknown
27852 (6)Conserved hypotheticalSA1388Unknown
52831 (2)Conserved hypotheticalSA1611Unknown
50661 (2)Conserved hypotheticalSA1684Unknown
49291 (2)Hypothetical proteinSA1889Unknown
55211 (2)Hypothetical proteinSA1928Unknown
25051 (2)Hypothetical proteinSA1944Unknown
15551 (2)AcetyltransferaseSA2161Unknown
91 (1)Hypothetical proteinSA2321Unknown
371 (1)Hypothetical proteinSA2343Unknown
39821 (2)Conserved hypotheticalSA2491Unknown
6441 (1)Hypothetical proteinSAS016Unknown
21051 (2)Conserved hypotheticalUnknown
22751 (1)Hypothetical proteinUnknown
4044 fnbA 1 (7)Fibronectin binding protein ASA2291Virulence factors
3636 fnbB 1 (3)Fibronectin binding protein BSA2291Virulence factors
4061 hla 1 (1)Alpha-toxinSA1007Virulence factorsYes
4578 hld 2 (2)Delta-hemolysinSAS065Virulence factorsYes
1928 hlgB 1 (5)Gamma-hemolysin component BSA2209Virulence factorsUpstream
1927 hlgC 1 (2)Gamma-hemolysin component CSA2208Virulence factorsYes
366 hsa 1 (X)Homolog of streptococcal hemagglutininSA2447Virulence factors
4374 map 3 (3)Map proteinSA2006Virulence factors
1175 pls 1 (2)Related to Pls and accumulation-associated proteins, LPXTG motifSA2284Virulence factors
2343 sdrc 2 (2)Surface protein (SdrC)Virulence factors
2037 set9 1 (4)Staphylococcal exotoxinSA0385Virulence factors
5329 splA 1 (2)Serine proteaseSA1631Virulence factorsYes
2929 splB 1 (1)Serine proteaseSA1630Virulence factorsUpstream
324 splD 1 (1)Serine proteaseSA1628Virulence factorsUpstream
327 splF 2 (2)Serine proteaseSA1627Virulence factorsUpstream
45232 (2)Phenol soluble modulin beta 2Virulence factors
  • a Designated S. aureusGeneChip ORF number.

  • b Previously described gene name.

  • c Many genes are represented on the S. aureus GeneChip in a redundant manner as full or partial fragments. Values are the number of times a gene transcript was identified as fitting a pattern (number of times the gene was either partially or completely represented on the GeneChip).

  • d Previously described gene product function.

  • e Corresponding designated S. aureusstrain N315 gene (20).

  • f Expected metabolic role.

  • g Yes, putative Sar box identified within the gene's promoter region. Upstream, the gene is expected to be part of an operon with a potential Sar box located in an upstream promoter region.