Table 3.

Oligonucleotides used in this study

NameSequenceDescription
TM289GAAGAATTCGAAAAAGAAGAATGACTTGGPclpP′-bgaBfusion
TM290GGAGGATCCTTTTGAGTTTTAATTTTGTTGGPclpP′-bgaBfusion
TM291GAAGAATTCCACGCTTGGTATCTTGAGATTCACPclpC′-bgaBfusion
TM292GGAGGATCCTCTCTTTAAACCTTGACCTTGPclpC′-bgaBfusion
AC6CTCCTCGAGCTTCCCTTTTCTATCTACCTCCtsR overproduction
AC7GGAGGATCCGTTTAAAGAGAGAGGTGGGTTTGTPxylA′-ctsRfusion
AC8GAAGAATTCAATAGTTCATCTTACTTCCCTPxylA′-ctsRfusion
AC9GGTGGTCTCCCATGAGATTTAAAAATACATCGGATCATACtsR overproduction
AC22TCGAAAAGAAGAATGACTTGGPclpPfootprint
AC23TCCTTTTGAGTTTTAATTTTGTTGGPclpPfootprint
AC24GAATTAGGCTTAGATAAGTAGPclpCfootprint
AC25CCAGATTGATCTAAAATCGCCPclpCfootprint
AC72CGTAAGAACGTTCTCCACGGCTTGTTTGPrimer extension on clpP
AC80ATTGAATTCATCGCAATGGAAATTTACGAACPclpE′-bgaBfusion
AC81AAAGGATCCATCTACCTCATTTCTTTCTTTAGCCPclpE′-bgaBfusion
AC82GTGAGAATTCTGCAGGCCAAGATTTGGCAGPgroE′-bgaBfusion
AC83TCTGGATCCCTCCATAATGAGATAGPgroE′-bgaBfusion
AC84GTTAGAATTCGCTTCTTGGGGTAT aphA3cassette
AC85TAGGGATCCAAATCTAGGTACTAA aphA3cassette
AC86TCCCCATGGACTTAGCGGTGGGATG ctsRdeletion
AC87ATCGAATTCCAAACCCACCTCTCTC ctsRdeletion
AC88AAGGGATCCAAGATGAACTATTCAAAAGC ctsRdeletion
AC89GTACCATGGCGTTGGCGTAAAGCC ctsRdeletion
AC90AAACCATGGAATGAATGTATCGAAAGTGCC clpCdeletion
AC91GTACGAATTCGTTGGCGTAAAGCC clpCdeletion
AC92TGGGGATCCGGATATTCGTTTTGACCAGG clpCdeletion
AC93ATGCCATGGCAAATTTTAACTGGCCTGC clpCdeletion
AC94TGACCATGGTTCCAGCTGCTAAAGTTGGC clpPdeletion
AC95TTTGAATTCTAATTTTGTTGGTCAAATG clpPdeletion
AC96ACTGGATCCGCGCCCAGGAAACACTTG clpPdeletion
AC97GCTACCATGGCAAGCGCCACAAACGATAG clpPdeletion
AC98CCTCCATGGTAAAATAGTAACGATAAG clpEdeletion
AC99CCTGAATTCTTTAAAGGTCAAAAATAG clpEdeletion
AC100GCAAGGGATCCATTCAGATTAAATCTGCC clpEdeletion
AC101ATACCATGGATAATGCAAGATTCC clpEdeletion
AC112TTTTCATTGTAACAACTTCTCAAAGCPclpEfootprint
AC113CCATTGAGATTGGTGTAAAGATGPclpEfootprint
AC114GATTTGGCAGATTTGGTCTTGGPgroEfootprint
AC115CGGTCCCCTAATGGTTTCAACPgroEfootprint
AC124AGCCTGCAAGGACAAAGCCTCCPrimer extension on groE
MP122CGCGGATCCGGTGTAGACGTTAAACGTCC ssbB′-lucfusion
MP158GCCGCGAAGCTTCTCAGGATATTGCAGATAC ssbB′-lucfusion