Table 1.

Induction of class I (HrcA-dependent) heat shock genes

GenebMicroarray results (fold induction at time indicated)mRNA levels from slot blot analysisa
3 min10 min20 min5 min10 min15 min
hrcA 6.08.62.610.511.04.0
 grpE 3.15.82.27.07.02.0
 dnaKc 2.6 (2.5)5.5 (3.1)2.3 (2.3)6.06.53.0
 dnaJ 2.02.00.883.53.01.5
 yqeT 1.71.50.673.53.01.5
 yqeU 1.10.850.553.03.01.0
 yqeV 1.41.10.792.52.51.0
groEL 3.16.94.1
 groES 2.7
  • a Values were estimated to the nearest 0.5-fold. from a slot blot histogram (Fig. 2) (25).

  • b Downstream genes in operons are indented.

  • c Quantitative RT-PCR results are shown in parentheses.