TABLE 3.

Genes with altered transcript amounts in a ΔpurR::aad9 mutant compared to its isogenic purR+ parent

Functional group and R6 genome entry no.GeneAvg fold change in ΔpurR mutantaProtein
Purine gene cluster
    spr0045purC13.5Phosphoribosylaminoimidazole-succinocarboxamide synthetase
    spr0046purL26.2Phosphoribosylformylglycinamide synthetase
    spr0047purF12.5Amidophosphoribosyl transferase
    spr0048purM10.6Phosphoribosylaminoimidazole synthetase
    spr0049purN14.65′-Phosphoribosylglycinamide transformylase 1
    spr0050vanZ18.6Teicoplanin resistance protein
    spr0051purH23.4Phosphoribosylaminoimidazolecarboxamide formyltransferase
    spr0052purD8.7Phosphoribosylglycinamide synthetase
    spr0053purE5.0Phosphoribosylaminoimidazole carboxylase, catalytic subunit
    spr0054purK12.4Phosphoribosyl glucinamide formyltransferase
    spr0055spr00554.7Hypothetical protein
    spr0056purB3.9Adenylosuccinate lyase
Purine transport and salvage
    spr0264spr02646.5Guanine/hypoxanthine transporter?
    spr1128guaC5.5GMP reductase
    spr1662xpt3.0Xanthine phosphoribosyltransferase
    spr1663pbuX3.2Nucleobase:cation symporter for xanthine
Folate metabolism
    spr0266sulA2.5Dihydropteroate synthase
    spr0267sulB2.6Dihydrofolate synthetase
    spr0268sulC2.5GTP cyclohydrolase
    spr0269sulD2.1Aldolase-pyrophosphokinase
    spr1109fhs2.2Formate-tetrahydrofolate ligase
Hypothetical and other
    spr0105spr01052.6Hypothetical transporter-truncation
    spr0265spr02656.5Conserved hypothetical protein
    spr0639copY2.7COPAB ATPases metal-fist-type repressor
    spr0641ctpA2.2P-type ATPase, probable copper transporter
    spr1440spr14402.5Conserved hypothetical protein
    spr1441oxlT3.6Major facilitator:oxalate:formate antiporter
    spr1792lysA−3.9Diaminopimelate decarboxylase
    spr1793purR−27.2Repressor of purine biosynthetic genes
  • a Microarray analyses were performed as described in Materials and Methods on bacteria grown in CDM to mid-exponential phase. Only genes whose transcript amounts showed a relative change of ≥2-fold in three independent experiments are listed. A negative value indicates a decrease in average relative transcript amount.