TABLE 1.

Primers used in this study

PrimerPrimer sequence (5′ → 3′)aCoordinates
SpoT F1TTT TTG TCG ACG AGC TC∗C GGT AGT GGA TGA GC195219-195233b
SpoT F2TT GCA CAT TTA ATC AAG ATA AAT G195713-195736b
SpoT R1CGG ATT AGC TGG CAC AT196044-196028b
SpoT R2TTT TTC TGC AG∗G GTT TTG TTC TAA AGA ATC ATC C198165-198142b
RT F −25GAA AAG AGA GCA AGG GCG CTG ATA195657-195680b
RT F −50ACA TTT AAA AAC AAT TTA CGA TCT T195632-195656b
RT R +800TAA GTT TGT TTG TTC TTG TTT GCA T196507-196483b
RT R +850TTT TGT TTT TTG CAA ATT ATT CTT A196556-196532b
RT R +950TTG ATA TTT ATT TTC TTT TGG GCT T196656-196632b
OspA F1GGG AAT AGG TCT AAT ATT AGC CTT9411-9434c
OspA R1CTG CTG ACC CCT CTA ATT TGG TGC C10171-10147c
  • a Bold letters indicate recognition sites for the restriction endonucleases used. SpoT F1 includes SacI and SalI sites. SpoT R2 contains a PstI site. ∗ indicates the start of the B. burgdorferi-specific sequence.

  • b Coordinates from B. burgdorferi main chromosome.

  • c Coordinates from B. burgdorferi linear plasmid 54 (lp54).