Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionSource or reference
    Escherichia coli
        XL-1 BluerecA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIq lacZM15 Tn10]Stratagene
        DH10BmcrA Δ(mrr hsdRMS mcrBC) φ80d lacZM15 ΔlacX74 deoR recA1 araD139 Δ(ara leu)7697 galU galK rpsL endA1 nupGBethesda Research Laboratories, Inc.
    Bacillus subtilis
        1012leuA8 metB5 trpC2 hsrM123
        WW01ftsH::erm derivative of 101236
        ED04ftsH::tet in 10129
        MP01ftsH::cat in 10129
        sigWsigW::neo allele in 101237
        ΔpbpEΔpbpE::erm allele isolated from PS1805 and transformed into 101221
        BFS233pMUTIN inserted into yuaF; promoter of yuaF fused to lacZ27
        MSW01pX2-ftsHThis work
        SZ01ΔpbpE::erm ftsH::catThis work
        SZ06ΔpbpE::erm amyE::PxylA-pbpE ftsH+ in 1012This work
        SZ08ΔsigW::neo pX2-ftsHThis work
        SZ09ΔpbpE::erm amyE::PxylA-pbpE ftsH::tet in 1012This work
        TW51amyE::PyuaF-lacZ; derived from pPyuaF-bgaB, with bgaB replaced by lacZThis work; PyuaF-bgaB from reference 37
        TW52amyE::PyuaF-lacZ ftsH::catThis work
    pQE303.5-kb vector allowing overproduction of proteins with six N-terminal histidine residues32
    pXCarries xylose-regulatable expression cassette for insertion at the amyE locus14
    pX2Carries xylose-regulatable expression cassette to be fused in front of the gene of interest18