TABLE 2.

Potential TAT substrates of A. tumefaciensa

Classification and proteinSequence from N terminusLocation and accession no.b
ABC transporters
    Periplasmic substrate binding proteins
        Substrate binding protein (sugar)cMDRRSFIRKAGAVGAGVTATALAAPAIAQENCC, gi_17934165
        Substrate binding protein (sugar)cMTHSLLNPTRRAFLAGTAAIGGSAMLGIRPACC, gi_17936386
        Substrate binding protein (molybdate)cMRLMRRSFFKTIAAATSFWMAASFVAVPACC, gi_17936436
        Substrate binding protein (thiamine)cMRRRLFLNSVIAASLFLPGLAAAQDKPCC, gi_17936589
        Substrate binding protein (sugar)cMIDRRGFLVTAALAGLGAAAGGFRAMAALC, gi_17936817
        Substrate binding protein (oligopeptide)cMTIFNQTTRRGFLLASVALGVVSLPGLKLNALC, gi_17937141
        Substrate binding protein (oligopeptide)cMTFSRRQFHKIALAAGAFVALPGGSFAAALC, gi_17937150
        Substrate binding protein (ferrichrome)cMSPLWGRREFLGMLAASAFAGTARAKQTPRILC, gi_17937719
        Substrate binding protein (spermidine/putrescine)cMTDITNSNKGLSRRGLLKAGAAATGAAIGSGLC, gi_17936799
        Substrate binding protein (C4 dicarboxylate)cMKDNPGRRQFLSGGVLAGAAAAASTLATPAIAt, gi_17938853
        Substrate binding proteinMQDFNITRRRFGLLAAGAAVAAAVPFAARAAAt, gi_17938994
        Substrate binding proteincMVFNRREFLKTAATASAALALPALGGRAFAt, gi_17939113
        Substrate binding protein (peptide)MIKLSRRTFNAALGIGALGTAIPGISFAQTTi, gi_17937206
        Substrate binding protein (agrocinopine)cMLKTNRRNFMMGTAAIAVASTAGAKFTFAQTi, gi_17939272
    Membrane-spanning proteins
        Membrane-spanning protein (sugar)MRRMFDRNIHAYLFLLPWLIGFFGLTLC, gi_17936824
        Membrane-spanning proteincMSVITIEPAITPRRRAFTPEDRIFVSIGLYGLC, gi_17938260
Redox proteins
    Ubiquinol-cytochrome c reductase FeS subunitcMSEHVTNHDASGEPTRRDFLYLVTGMAGAVGCC, gi_17936122
    Formate dehydrogenase H alpha subunitMFLTNGKRRQPGTRRQFPGKRTMSKTDFIGKCC, gi_17935447
    Periplasmic nitrate reductase large subunitcMSSELTRRNLLKMKAHAAGIAAATAGIALPALC, gi_17938097
    Periplasmic nitrate reductase ferredoxin-like proteinMTMDVSLSRRDFLRGGQKREMRICPPGVALSLC, gi_17938095
Others
    Epoxide hydrolaseMLRRTFIALAAQLVVYGSVKRALSHEACC, gi_17935704
    Endo-1,4-beta-xylanasecMRIGRRNFLGGAASAGLLHAVAGPAFAAQKKCC, gi_17936253
    Periplasmic mannitol-binding proteincMDRRSFMKKGALAGAAATALAAPAIAQLC, gi_17937076
    Guanylate kinasecMVPVNNSPVTIARRGLMLVISSPSGAGKSTICC, gi_17935008
    2′,3′-Cyclic-nucleotide 2′-phosphodiesteraseMSSLLALHPITRRSLLGGIAASSALVLLHPFCC, gi_17935115
    Metallo-beta-lactamase superfamily proteincMPLLSRRTFASALPLGLLAGGLIGVGKAISAt, gi_17939129
  • a This list is a subset of 77 possible substrates, of which 30 are putative periplasmic ligand binding proteins, identified by screening for RRxF or RRxL motifs in signal sequences.

  • b CC, circular chromosome; LC, linear chromosome, At, pAtC58; Ti, pTiA6NC.

  • c The protein was also identified with the TATFIND algorithm. See text for details.