TABLE 3.

Effects of a tatC mutation on virB-dependent conjugal transfera

DonorRecipientNo. of TcsNo. of donorsNo. of recipientsTcs per donorTcs per recipient
PC1000b (pML122Genr)A348 (Spcr)01.3 × 107NDc<7.7 × 10−8ND
PC1000 (pML122Genr)PC2000 (Kanr)04.8 × 1085.6 × 108<2.1 × 10−9<1.8 × 10−9
A348 (pML122Genr)A348 (Spcr)6.3 × 1044.6 × 1081.8 × 1091.4 × 10−43.5 × 10−5
PC2000 (pML122Genr)A348 (Spcr)2.1 × 1021.9 × 106ND1.1 × 10−4ND
A348 (pML122Genr)PC2000 (Kanr)1.1 × 1041.4 × 1099.3 × 1077.8 × 10−61.2 × 10−4
  • a Input donor and recipient cells were mixed in a 1:1 ratio and incubated for 3 days on ABIM (vir induction medium with 200 mM acetosyringone) at 18°C. The data shown are from a single experiment. Experiments were repeated three times, with <5% standard variation. Transconjugants (Tcs) and output donor and recipient cell numbers were calculated, and conjugation frequencies are reported as the number of Tcs per output donor or recipient cell.

  • b PC1000, a virB operon deletion mutant, served as a negative control for these experiments.

  • c ND, not determined.