TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionSource or reference
Strains
    J96Uropathogenic E. coli21
    DS17Uropathogenic E. coli38
    AKP013J96 barA::KanrThis study
    AKP159J96 uvrY::KanrThis study
    AKP221DS17 barA::KanrThis study
    AKP168DS17 uvrY::KanrThis study
    MC4100StrrAmerican Type Culture Collection
    AKP014MC4100 barA::Kanr30
    AKP023MC4100 uvrY::Camr30
    MG1655Michael Cashel
    RG1-B MG1655csrB::Camr17
    AKP199MG1655 barA::Kanr (from AKP014)This study
    AKP200MG1655 uvrY::Camr (from AKP023)This study
    AKP 287MG1655 barA::Kanr csrB::Camr (from RG1-B MG1655)This study
    XL-1 BlueTetrStratagene
Plasmids
    pCA9505Carries uvrY gene, Ampr26
    pCA9505-MluIEncodes truncated UvrY protein, AmprThis study
    pGEM-T easyT-cloning vector, AmprPromega
    pUC4KContains kanamycin resistance cassetteAmersham Pharmacia Biotech
    pKO3Plasmid used in allelic replacement23
    pCVD422Plasmid used in allelic replacement11